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Journal of Virology, January 1999, p. 834-838, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Feline Calicivirus Capsid Protein Expression and Capsid Assembly in Cultured Feline Cells

Klaus Geissler,1 Karla Schneider,1 Andrea Fleuchaus,2 Colin R. Parrish,3 Gerd Sutter,2 and Uwe Truyen1,*

Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig Maximilians University Munich, 80539 Munich,1 and Institute of Molecular Virology, GSF---National Center of Environmental and Health Research, 85764 Munich-Neuherberg,2 Germany, and James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 148533

Received 14 April 1998/Accepted 12 October 1998

The capsid protein of feline calicivirus (FCV) was expressed by using plasmids containing cytomegalovirus, simian virus 40, or T7 promoters. The strongest expression was achieved with the T7 promoter and coinfection with vaccinia virus expressing the T7 RNA polymerase (MVA/T7pol). The FCV precursor capsid protein was processed to the mature-size protein, and these proteins were assembled in to virus-like particles.

* Corresponding author. Mailing address: Ludwig Maximilians University Munich, Veterinaerstr. 13, 80539 Munich, Germany. Phone: 49-89-2180-2535. Fax: 49-89-2180-2155. E-mail: UWE.TRUYEN{at}LRZ.UNI-MUENCHEN.DE.

Journal of Virology, January 1999, p. 834-838, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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