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Journal of Virology, January 1999, p. 377-387, Vol. 73, No. 1
Institut de Biologie, EP CNRS 525, Institut
Pasteur de Lille, 59021 Lille Cedex, France,1
and
Department of Cell Biology, Yale University School of
Medicine, New Haven, Connecticut 065102
Received 9 July 1998/Accepted 8 October 1998
Herpes simplex virus (HSV) and varicella-zoster virus (VZV) are two
pathogenic human alphaherpesviruses whose intracellular assembly is
thought to follow different pathways. VZV presumably acquires its
envelope in the trans-Golgi network (TGN), and it has
recently been shown that its major envelope glycoprotein, VZV-gE,
accumulates in this compartment when expressed alone. In contrast, the
envelopment of HSV has been proposed to occur at the inner nuclear
membrane, although to which compartment the gE homolog (HSV-gE) is
transported is unknown. For this reason, we have studied the
intracellular traffic of HSV-gE and have found that this glycoprotein
accumulates at steady state in the TGN, both when expressed from cloned
cDNA and in HSV-infected cells. In addition, HSV-gE cycles between the
TGN and the cell surface and requires a conserved tyrosine-containing
motif within its cytoplasmic tail for proper trafficking. These results
show that VZV-gE and HSV-gE have similar intracellular trafficking
pathways, probably reflecting the presence of similar sorting signals
in the cytoplasmic domains of both molecules, and suggest that the respective viruses, VZV and HSV, could use the same subcellular organelle, the TGN, for their envelopment.
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Intracellular Traffic of Herpes Simplex Virus Glycoprotein gE:
Characterization of the Sorting Signals Required for Its
trans-Golgi Network Localization

and
*
Corresponding author. Mailing address: Institut de
Biologie, EP CNRS 525, Institut Pasteur de Lille, 1 Rue du Professeur
Calmette, 59021 Lille Cedex, France. Phone: (33)320871027. Fax:
(33)320871019. E-mail:
Bernard.Hoflack{at}pasteur-lille.fr.
Present address: Biozentrum der Universität Basel, 4056 Basel, Switzerland.
Present address: Medizinische Hochschule Hannover, Institut
für Biochemie, D-30623 Hannover, Germany.
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