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Journal of Virology, January 1999, p. 377-387, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Intracellular Traffic of Herpes Simplex Virus Glycoprotein gE: Characterization of the Sorting Signals Required for Its trans-Golgi Network Localization

Agustín Alconada,1,dagger Ulrike Bauer,1 Beate Sodeik,2,Dagger and Bernard Hoflack1,*

Institut de Biologie, EP CNRS 525, Institut Pasteur de Lille, 59021 Lille Cedex, France,1 and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 065102

Received 9 July 1998/Accepted 8 October 1998

Herpes simplex virus (HSV) and varicella-zoster virus (VZV) are two pathogenic human alphaherpesviruses whose intracellular assembly is thought to follow different pathways. VZV presumably acquires its envelope in the trans-Golgi network (TGN), and it has recently been shown that its major envelope glycoprotein, VZV-gE, accumulates in this compartment when expressed alone. In contrast, the envelopment of HSV has been proposed to occur at the inner nuclear membrane, although to which compartment the gE homolog (HSV-gE) is transported is unknown. For this reason, we have studied the intracellular traffic of HSV-gE and have found that this glycoprotein accumulates at steady state in the TGN, both when expressed from cloned cDNA and in HSV-infected cells. In addition, HSV-gE cycles between the TGN and the cell surface and requires a conserved tyrosine-containing motif within its cytoplasmic tail for proper trafficking. These results show that VZV-gE and HSV-gE have similar intracellular trafficking pathways, probably reflecting the presence of similar sorting signals in the cytoplasmic domains of both molecules, and suggest that the respective viruses, VZV and HSV, could use the same subcellular organelle, the TGN, for their envelopment.

* Corresponding author. Mailing address: Institut de Biologie, EP CNRS 525, Institut Pasteur de Lille, 1 Rue du Professeur Calmette, 59021 Lille Cedex, France. Phone: (33)320871027. Fax: (33)320871019. E-mail: Bernard.Hoflack{at}pasteur-lille.fr.

dagger Present address: Biozentrum der Universität Basel, 4056 Basel, Switzerland.

Dagger Present address: Medizinische Hochschule Hannover, Institut für Biochemie, D-30623 Hannover, Germany.

Journal of Virology, January 1999, p. 377-387, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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