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Journal of Virology, January 1999, p. 1-10, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Detailed Analysis of the Humoral Immune Response to a Protective Vaccine

Paola Mazzetti,1 Simone Giannecchini,1 Daniela Del Mauro,1 Donatella Matteucci,1 Pietro Portincasa,2 Antonio Merico,1 Carlo Chezzi,2 and Mauro Bendinelli1,*

Department of Biomedicine and Retrovirus Center, University of Pisa, Pisa,1 and Institute of Microbiology, University of Parma, Parma,2 Italy

Received 8 July 1998/Accepted 18 September 1998

The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechanisms responsible for protection are elusive. In previous studies we showed that cats immunized with a vaccine consisting of fixed infected cells were protected or unprotected against cell-free or cell-associated FIV challenge depending on the time interval between completion of vaccination and challenge. In an attempt to define possible humoral immune correlates of protection, selected sera harvested at the times of challenge from such cats were examined for anti-FIV-antibody titers and properties by using binding and functional immunological assays. Binding assays included quantitative Western blotting, enzyme-linked tests for antibodies to FIV glycoproteins and immunodominant linear epitopes, and tests for measuring conformation dependence and avidity of anti-viral-envelope antibodies. Functional assays included virus neutralization performed with two different cell substrates, complement- and antibody-dependent virolysis, blocking of reverse transcriptase, and an assay that measured the ability of sera to prevent FIV growth in cocultures of infected and uninfected cells. Despite the wide spectrum of parameters investigated, no correlation between vaccine-induced protection and the humoral parameters measured was noted.

* Corresponding author. Mailing address: Dipartimento di Biomedicina, Università di Pisa, Via San Zeno 37, I-56127 Pisa, Italy. Phone: 39-050-553562. Fax: 39-050-556455. E-mail: bendinelli{at}biomed.unipi.it.

Journal of Virology, January 1999, p. 1-10, Vol. 73, No. 1
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Giannecchini, S., Isola, P., Sichi, O., Matteucci, D., Pistello, M., Zaccaro, L., Del Mauro, D., Bendinelli, M. (2002). AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Failure To Protect and Possible Enhancement of Challenge Infection by Four Cell-Based Vaccines Prepared with Autologous Lymphoblasts. J. Virol. 76: 6882-6892 [Abstract] [Full Text]  
  • Giannecchini, S., Del Mauro, D., Matteucci, D., Bendinelli, M. (2001). AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Reevaluation of Neutralizing Antibody Levels Elicited by a Protective and a Nonprotective Vaccine after Removal of Antisubstrate Cell Antibodies. J. Virol. 75: 4424-4429 [Abstract] [Full Text]  
  • Matteucci, D., Poli, A., Mazzetti, P., Sozzi, S., Bonci, F., Isola, P., Zaccaro, L., Giannecchini, S., Calandrella, M., Pistello, M., Specter, S., Bendinelli, M. (2000). Immunogenicity of an Anti-Clade B Feline Immunodeficiency Fixed-Cell Virus Vaccine in Field Cats. J. Virol. 74: 10911-10919 [Abstract] [Full Text]  


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