This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vines, A.
Right arrow Articles by Kawaoka, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vines, A.
Right arrow Articles by Kawaoka, Y.

 Previous Article  |  Next Article 

Journal of Virology, September 1998, p. 7626-7631, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Role of Influenza A Virus Hemagglutinin Residues 226 and 228 in Receptor Specificity and Host Range Restriction

Angela Vines,1,dagger Krisna Wells,1,2 Mikhail Matrosovich,1 Maria R. Castrucci,3 Toshihiro Ito,4 and Yoshihiro Kawaoka1,2,5,*

Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 381051; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin---Madison, Madison, Wisconsin 537062; Department of Virology, Instituto Superiore di Sanita, 00161 Rome, Italy3; Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Tottori 680, Japan4; and Department of Pathology, University of Tennessee, Memphis, Memphis, Tennessee 381635

Received 13 March 1998/Accepted 19 May 1998

Influenza A viruses can be isolated from a variety of animals, but their range of hosts is restricted. For example, human influenza viruses do not replicate in duck intestine, the major replication site of avian viruses in ducks. Although amino acids at positions 226 and 228 of hemagglutinin (HA) of the H3 subtype are known to be important for this host range restriction, the contributions of specific amino acids at these positions to restriction were not known. Here, we address this issue by generating HAs with site-specific mutations of a human virus that contain different amino acid residues at these positions. We also let ducks select replication-competent viruses from a replication-incompetent virus containing a human virus HA by inoculating animals with 1010.5 50% egg infectious dose of the latter virus and identified a mutation in the HA. Our results showed that the Ser-to-Gly mutation at position 228, in addition to the Leu-to-Gln mutation at position 226 of the HA of the H3 subtype, is critical for human virus HA to support virus replication in duck intestine.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin---Madison, 2015 Linden Dr. West, Madison, WI 53706. Phone: (608) 265-4925. Fax: (608) 265-5622. E-mail: kawaokay{at}svm.vetmed.wisc.edu.

dagger Present address: Department of Biology, Spelman College, Atlanta, GA 30314-4399.

Journal of Virology, September 1998, p. 7626-7631, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, C., Hatta, M., Watanabe, S., Neumann, G., Kawaoka, Y. (2008). Compatibility among Polymerase Subunit Proteins Is a Restricting Factor in Reassortment between Equine H7N7 and Human H3N2 Influenza Viruses. J. Virol. 82: 11880-11888 [Abstract] [Full Text]  
  • Keleta, L., Ibricevic, A., Bovin, N. V., Brody, S. L., Brown, E. G. (2008). Experimental Evolution of Human Influenza Virus H3 Hemagglutinin in the Mouse Lung Identifies Adaptive Regions in HA1 and HA2. J. Virol. 82: 11599-11608 [Abstract] [Full Text]  
  • Meisner, J., Szretter, K. J., Bradley, K. C., Langley, W. A., Li, Z.-N., Lee, B.-J., Thoennes, S., Martin, J., Skehel, J. J., Russell, R. J., Katz, J. M., Steinhauer, D. A. (2008). Infectivity Studies of Influenza Virus Hemagglutinin Receptor Binding Site Mutants in Mice. J. Virol. 82: 5079-5083 [Abstract] [Full Text]  
  • Kerby, M. B., Freeman, S., Prachanronarong, K., Artenstein, A. W., Opal, S. M., Tripathi, A. (2008). Direct Sequence Detection of Structured H5 Influenza Viral RNA. J. Mol. Diagn. 10: 225-235 [Abstract] [Full Text]  
  • Wang, Q., Tian, X., Chen, X., Ma, J. (2007). Structural basis for receptor specificity of influenza B virus hemagglutinin. Proc. Natl. Acad. Sci. USA 104: 16874-16879 [Abstract] [Full Text]  
  • Crescenzo-Chaigne, B., van der Werf, S. (2007). Rescue of Influenza C Virus from Recombinant DNA. J. Virol. 81: 11282-11289 [Abstract] [Full Text]  
  • Auewarakul, P., Suptawiwat, O., Kongchanagul, A., Sangma, C., Suzuki, Y., Ungchusak, K., Louisirirotchanakul, S., Lerdsamran, H., Pooruk, P., Thitithanyanont, A., Pittayawonganon, C., Guo, C.-T., Hiramatsu, H., Jampangern, W., Chunsutthiwat, S., Puthavathana, P. (2007). An Avian Influenza H5N1 Virus That Binds to a Human-Type Receptor. J. Virol. 81: 9950-9955 [Abstract] [Full Text]  
  • Yang, Z.-Y., Wei, C.-J., Kong, W.-P., Wu, L., Xu, L., Smith, D. F., Nabel, G. J. (2007). Immunization by Avian H5 Influenza Hemagglutinin Mutants with Altered Receptor Binding Specificity. Science 317: 825-828 [Abstract] [Full Text]  
  • Wan, H., Perez, D. R. (2007). Amino Acid 226 in the Hemagglutinin of H9N2 Influenza Viruses Determines Cell Tropism and Replication in Human Airway Epithelial Cells. J. Virol. 81: 5181-5191 [Abstract] [Full Text]  
  • Ibricevic, A., Pekosz, A., Walter, M. J., Newby, C., Battaile, J. T., Brown, E. G., Holtzman, M. J., Brody, S. L. (2006). Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells.. J. Virol. 80: 7469-7480 [Abstract] [Full Text]  
  • Thompson, C. I., Barclay, W. S., Zambon, M. C., Pickles, R. J. (2006). Infection of human airway epithelium by human and avian strains of influenza a virus.. J. Virol. 80: 8060-8068 [Abstract] [Full Text]  
  • Crawford, P. C., Dubovi, E. J., Castleman, W. L., Stephenson, I., Gibbs, E. P. J., Chen, L., Smith, C., Hill, R. C., Ferro, P., Pompey, J., Bright, R. A., Medina, M.-J., Influenza Genomics Group, , Johnson, C. M., Olsen, C. W., Cox, N. J., Klimov, A. I., Katz, J. M., Donis, R. O. (2005). Transmission of Equine Influenza Virus to Dogs. Science 310: 482-485 [Abstract] [Full Text]  
  • Nokhbeh, M. R., Hazra, S., Alexander, D. A., Khan, A., McAllister, M., Suuronen, E. J., Griffith, M., Dimock, K. (2005). Enterovirus 70 Binds to Different Glycoconjugates Containing {alpha}2,3-Linked Sialic Acid on Different Cell Lines. J. Virol. 79: 7087-7094 [Abstract] [Full Text]  
  • Lee, C.-W., Suarez, D. L., Tumpey, T. M., Sung, H.-W., Kwon, Y.-K., Lee, Y.-J., Choi, J.-G., Joh, S.-J., Kim, M.-C., Lee, E.-K., Park, J.-M., Lu, X., Katz, J. M., Spackman, E., Swayne, D. E., Kim, J.-H. (2005). Characterization of Highly Pathogenic H5N1 Avian Influenza A Viruses Isolated from South Korea. J. Virol. 79: 3692-3702 [Abstract] [Full Text]  
  • Harvey, R., Martin, A. C. R., Zambon, M., Barclay, W. S. (2004). Restrictions to the Adaptation of Influenza A Virus H5 Hemagglutinin to the Human Host. J. Virol. 78: 502-507 [Abstract] [Full Text]  
  • Gagneux, P., Cheriyan, M., Hurtado-Ziola, N., van der Linden, E. C. M. B., Anderson, D., McClure, H., Varki, A., Varki, N. M. (2003). Human-specific Regulation of {alpha}2-6-linked Sialic Acids. J. Biol. Chem. 278: 48245-48250 [Abstract] [Full Text]  
  • Landolt, G. A., Karasin, A. I., Phillips, L., Olsen, C. W. (2003). Comparison of the Pathogenesis of Two Genetically Different H3N2 Influenza A Viruses in Pigs. J. Clin. Microbiol. 41: 1936-1941 [Abstract] [Full Text]  
  • Skiadopoulos, M. H., Schmidt, A. C., Riggs, J. M., Surman, S. R., Elkins, W. R., St. Claire, M., Collins, P. L., Murphy, B. R. (2002). Determinants of the Host Range Restriction of Replication of Bovine Parainfluenza Virus Type 3 in Rhesus Monkeys Are Polygenic. J. Virol. 77: 1141-1148 [Abstract] [Full Text]  
  • Chin, P. S., Hoffmann, E., Webby, R., Webster, R. G., Guan, Y., Peiris, M., Shortridge, K. F. (2002). Molecular Evolution of H6 Influenza Viruses from Poultry in Southeastern China: Prevalence of H6N1 Influenza Viruses Possessing Seven A/Hong Kong/156/97 (H5N1)-Like Genes in Poultry. J. Virol. 76: 507-516 [Abstract] [Full Text]  
  • Massin, P., van der Werf, S., Naffakh, N. (2001). Residue 627 of PB2 Is a Determinant of Cold Sensitivity in RNA Replication of Avian Influenza Viruses. J. Virol. 75: 5398-5404 [Abstract] [Full Text]  
  • Ito, T., Suzuki, Y., Suzuki, T., Takada, A., Horimoto, T., Wells, K., Kida, H., Otsuki, K., Kiso, M., Ishida, H., Kawaoka, Y. (2000). Recognition of N-Glycolylneuraminic Acid Linked to Galactose by the alpha 2,3 Linkage Is Associated with Intestinal Replication of Influenza A Virus in Ducks. J. Virol. 74: 9300-9305 [Abstract] [Full Text]  
  • Matrosovich, M., Tuzikov, A., Bovin, N., Gambaryan, A., Klimov, A., Castrucci, M. R., Donatelli, I., Kawaoka, Y. (2000). Early Alterations of the Receptor-Binding Properties of H1, H2, and H3 Avian Influenza Virus Hemagglutinins after Their Introduction into Mammals. J. Virol. 74: 8502-8512 [Abstract] [Full Text]  
  • Wagner, R., Wolff, T., Herwig, A., Pleschka, S., Klenk, H.-D. (2000). Interdependence of Hemagglutinin Glycosylation and Neuraminidase as Regulators of Influenza Virus Growth: a Study by Reverse Genetics. J. Virol. 74: 6316-6323 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»