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Journal of Virology, September 1998, p. 7615-7619, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Crystallization and Preliminary X-Ray Analysis of Rotavirus Protein VP6

Isabelle Petitpas,1,2 Jean Lepault,3 Patrice Vachette,4 Annie Charpilienne,5 Magali Mathieu,1 Evelyne Kohli,2 Pierre Pothier,2 Jean Cohen,5 and Félix A. Rey1,*

Laboratoire d'Enzymologie et Biochimie Structurales, CNRS UPR 9063,1 and Centre de Génétique Moléculaire, CNRS-UPR 9061,3 91198 Gif-sur-Yvette Cedex, Laboratoire de Microbiologie Médicale et Moléculaire, UFR Médecine et Pharmacie, Université de Bourgogne, 21000 Dijon,2 Laboratoire pour l'Utilisation du Rayonnement Electromagnétique (LURE, CNRS-CEA-MENRES), Centre Universitaire Paris-Sud, 91405 Orsay Cedex,4 and Laboratoire de Virologie et Immunologie Moléculaires, INRA, CRJ, Domaine de Vilvert, 78350 Jouy-en-Josas,5 France

Received 23 March 1998/Accepted 19 May 1998

As a first step to gain insight into the structure of the rotavirus virion at atomic resolution, we report here the expression, purification, and crystallization of recombinant rotavirus protein VP6. This protein has the property of polymerizing in the form of tubular structures in solution which have hindered crystallization thus far. Using a combination of electron microscopy and small-angle X-ray scattering, we found that addition of Ca2+ at concentrations higher than 100 mM results in depolymerization of the tubes, leading to an essentially monodisperse solution of trimeric VP6 even at high protein concentrations (higher than 10 mg/ml), thereby enabling us to search for crystallization conditions. We have thus obtained crystals of VP6 which diffract to better than 2.4 Å resolution and belong to the cubic space group P4132 with a cell dimension a of 160 Å. The crystals contain a trimer of VP6 lying along the diagonal of the cubic unit cell, resulting in one VP6 monomer per asymmetric unit and a solvent content of roughly 70%.

* Corresponding author. Mailing address: Laboratoire d'Enzymologie et Biochimie Structurales, CNRS UPR 9063, 1, Ave. de la Terrasse Bât. 34, 91198 Gif-sur-Yvette Cedex, France. Phone: (33) 169 823 470. Fax: (33) 169 823 129. E-mail: rey{at}lebs.cnrs-gif.fr.

Journal of Virology, September 1998, p. 7615-7619, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.