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Journal of Virology, September 1998, p. 7598-7602, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Trophoblast Cells Are Permissive to the Complete Replicative Cycle of Human Cytomegalovirus

G. Halwachs-Baumann,1 M. Wilders-Truschnig,1 G. Desoye,2 T. Hahn,2 L. Kiesel,3 K. Klingel,4 P. Rieger,5 G. Jahn,6 and C. Sinzger6,*

Department of Laboratory Medicine1 and Department of Obstetrics and Gynecology,2 University of Graz, A-8036 Graz, Department of Obstetrics and Gynecology,3 Department of Pathology,4 and Department of Medical Virology,6 University of Tübingen, D-72076 Tübingen, and Department of Pathology, University of Heidelberg, D-69120 Heidelberg,5 Germany

Received 13 April 1998/Accepted 20 May 1998

Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast culture supernatant, and cell-to-cell spread of cytomegalovirus from infected trophoblasts to uninfected fibroblasts. These observations indicate that infected trophoblasts may be involved in maternofetal transmission of human cytomegalovirus.

* Corresponding author. Mailing address: Abt. Med. Virologie, Universität Tübingen, Calwerstrasse 7/6, D-72076 Tübingen, Germany. Phone: 49 7071 2987459. Fax: 49 7071 295790. E-mail: christian.sinzger{at}med.uni-tuebingen.de.

Journal of Virology, September 1998, p. 7598-7602, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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