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Journal of Virology, September 1998, p. 7476-7483, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Recognition of Herpes Simplex Virus Type 2 Tegument Proteins by CD4 T Cells Infiltrating Human Genital Herpes Lesions

David M. Koelle,1,2,3,* Jeannine M. Frank,2,3 Matthew L. Johnson,2 and William W. Kwok4

Department of Medicine1 and Department of Laboratory Medicine,2 University of Washington, Seattle, Washington 98105; Fred Hutchinson Cancer Research Center, Seattle, Washington 981043; and Virginia Mason Research Center, Seattle, Washington 981014

Received 16 March 1998/Accepted 19 May 1998

The local cellular immune response to herpes simplex virus (HSV) is important in the control of recurrent HSV infection. The antiviral functions of infiltrating CD4-bearing T cells may include cytotoxicity, inhibition of viral growth, lymphokine secretion, and support of humoral and CD8 responses. The antigens recognized by many HSV-specific CD4 T cells localizing to genital HSV-2 lesions are unknown. T cells recognizing antigens encoded within map units 0.67 to 0.73 of HSV DNA are frequently recovered from herpetic lesions. Expression cloning with this region of DNA now shows that tegument protein VP22 and the viral dUTPase, encoded by genes UL49 and UL50, respectively, are T-cell antigens. Separate epitopes in VP22 were defined for T-cell clones from each of three patients. Reactivity with the tegument protein encoded by UL21 was identified for an additional patient. Three new epitopes were identified in VP16, a tegument protein associated with VP22. Some tegument-specific CD4 T-cell clones exhibited cytotoxic activity against HSV-infected cells. These results suggest that herpes simplex tegument proteins are processed for antigen presentation in vivo and are possible candidate compounds for herpes simplex vaccines.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, Room D3-100, 1100 Fairview Ave. North, P.O. Box 19024, Seattle, WA 98109. Phone: (206) 667-6807. Fax: (206) 667-6707. E-mail: viralimm{at}u.washington.edu.

Journal of Virology, September 1998, p. 7476-7483, Vol. 72, No. 9
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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