This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xin, H.-W.
Right arrow Articles by Ding, S.-W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xin, H.-W.
Right arrow Articles by Ding, S.-W.

 Previous Article  |  Next Article 

J Virol, August 1998, p. 6956-6959, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Ilarviruses Encode a Cucumovirus-Like 2b Gene That Is Absent in Other Genera within the Bromoviridae

Hong-Wu Xin,1 Liang-Hui Ji,1 Simon W. Scott,2 Robert H. Symons,3 and Shou-Wei Ding1,*

Molecular Virology Laboratory, Institute of Molecular Agrobiology, National University of Singapore, Singapore 1176041; Department of Plant Pathology and Physiology, Clemson University, Clemson, South Carolina 29634-03772; and Department of Plant Science, Waite Campus, University of Adelaide, Glen Osmond, SA 5064, Australia3

Received 2 January 1998/Accepted 14 May 1998

We found that RNA 2 of the four ilarviruses sequenced to date encodes an additional conserved open reading frame (ORF), 2b, that overlaps the 3' end of the previously known ORF, 2a. A novel RNA species of 851 nucleotides was found to accumulate to high levels in plants infected with spinach latent virus (SpLV). Further analysis showed that RNA 4A is a subgenomic RNA of RNA 2 and encodes all of ORF 2b. Moreover, a protein species of the size expected for SpLV ORF 2b was translated in vitro from the RNA 4A-containing virion RNAs. The data support the suggestion that the SpLV 2b protein is translated in vivo. The 2b gene of ilarviruses, which is not encoded by alfamoviruses and bromoviruses, shares several features with the previously reported cucumovirus 2b gene; however, their encoded proteins share no detectable sequence similarities. The evolutionary origin of the 2b gene is discussed.

* Corresponding author. Mailing address: Molecular Virology Laboratory, Institute of Molecular Agrobiology, 1 Research Link, National University of Singapore, Singapore 117604. Phone: (65) 872 7090. Fax: (65) 872 7007. E-mail: dingsw{at}ima.org.sg.

J Virol, August 1998, p. 6956-6959, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Du, Z.-Y., Chen, F.-F., Liao, Q.-S., Zhang, H.-R., Chen, Y.-F., Chen, J.-S. (2007). 2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species. J. Gen. Virol. 88: 2596-2604 [Abstract] [Full Text]  
  • Lin, H.-X., Rubio, L., Smythe, A. B., Falk, B. W. (2004). Molecular Population Genetics of Cucumber Mosaic Virus in California: Evidence for Founder Effects and Reassortment. J. Virol. 78: 6666-6675 [Abstract] [Full Text]  
  • Roossinck, M. J. (2002). Evolutionary History of Cucumber Mosaic Virus Deduced by Phylogenetic Analyses. J. Virol. 76: 3382-3387 [Abstract] [Full Text]  
  • Shiel, P. J., Berger, P. H. (2000). The complete nucleotide sequence of apple mosaic virus (ApMV) RNA 1 and RNA 2: ApMV is more closely related to alfalfa mosaic virus than to other ilarviruses. J. Gen. Virol. 81: 273-278 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»