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J Virol, August 1998, p. 6902-6906, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differential Expression of Nur77 Family Members in Human T-Lymphotropic Virus Type 1-Infected Cells: Transactivation of the TR3/nur77 Gene by Tax Protein

Xiaolin Chen,1 Vladimir Zachar,1,2 Chawnshang Chang,3 Peter Ebbesen,1 and Xiangdong Liu1,*

Department of Virus and Cancer, Danish Cancer Society, DK-8000 Aarhus C, Denmark1; Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic2; and George H. Whipple Laboratory for Cancer Research, Departments of Pathology, Urology, and Biochemistry, University of Rochester, Rochester, New York 146423

Received 30 January 1998/Accepted 12 May 1998

We analyzed the differential expression and regulation of three members of the Nur77 transcription factor family by the human T-lymphotropic virus type 1 (HTLV-1) Tax protein. We have demonstrated that in both HTLV-1-infected cells and Tax-expressing JPX-9 cells, TR3/nur77 is highly expressed, whereas neither NOR-1 nor NOT expression is detectable. Transient transfection analysis further confirmed the Tax transactivation of the TR3/nur77 promoter but not the NOR-1 promoter in different cell types. Furthermore, expression of a luciferase reporter gene driven by the NGFI-B (rat homolog of TR3/Nur77) response element (NBRE) provided evidence that Tax-mediated transactivation resulted in the induction of a functional protein. Cotransfection assays with the TR3/nur77 promoter sequence or the NBRE binding motif together with a series of Tax mutants have shown that Tax-induced TR3/nur77 expression is mediated by CREB/ATF-related transcription factors.

* Corresponding author. Mailing address: Department of Virus and Cancer, Danish Cancer Society, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark. Phone: (45) 86 12 73 66. Fax: (45) 86 19 54 15. E-mail: liu{at}virus.aau.dk.

J Virol, August 1998, p. 6902-6906, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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