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J Virol, August 1998, p. 6822-6831, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

p53 Protein Is a Suppressor of Papillomavirus DNA Amplificational Replication

Dina Lepik,1 Ivar Ilves,1 Arnold Kristjuhan,2 Toivo Maimets,2 and Mart Ustav1,*

Department of Microbiology and Virology1 and Department of Cell Biology,2 Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, Tartu EE2400, Estonia

Received 9 March 1998/Accepted 12 May 1998

p53 protein was able to block human and bovine papillomavirus DNA amplificational replication while not interfering with Epstein-Barr virus oriP once-per-cell cycle replication. Oligomerization, intact DNA-binding, replication protein A-binding, and proline-rich domains of the p53 protein were essential for efficient inhibition, while the N-terminal transcriptional activation and C-terminal regulatory domains were dispensable for the suppressor activity of the p53 protein. The inhibition of replication was caused neither by the downregulation of expression of the E1 and E2 proteins nor by cell cycle block or apoptosis. Our data suggest that the intrinsic activity of p53 to suppress amplificational replication of the papillomavirus origin may have an important role in the virus life cycle and in virus-cell interactions.

* Corresponding author. Mailing address: Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University, 23 Riia St., Tartu EE2400, Estonia. Phone: 372-7-465047. Fax: 372-7-420286. E-mail: ustav{at}ebc.ee.

J Virol, August 1998, p. 6822-6831, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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