This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jamieson, B. D.
Right arrow Articles by Zack, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jamieson, B. D.
Right arrow Articles by Zack, J. A.

 Previous Article  |  Next Article 

J Virol, August 1998, p. 6520-6526, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

In Vivo Pathogenesis of a Human Immunodeficiency Virus Type 1 Reporter Virus

Beth D. Jamieson1 and Jerome A. Zack1,2,*

Division of Hematology-Oncology, Department of Medicine,1 and Department of Microbiology and Molecular Genetics,2 UCLA School of Medicine and UCLA AIDS Institute, Los Angeles, California 90095-1678

Received 5 March 1998/Accepted 12 May 1998

Our understanding of human immunodeficiency virus type 1 (HIV-1)-induced pathogenesis is hampered by the inability to detect HIV-1 gene expression in infected viable cells. In this report, we describe two HIV-1 reporter constructs that are replication competent and cytopathic in vivo. These constructs contain DNA regions of two different lengths that bear the cDNA for the murine heat-stable antigen in the vpr region of a CXCR4-tropic virus. We used the SCID-hu mouse model and these reporter viruses to perform detailed kinetic studies of HIV-1 infection of human thymocytes in vivo. We document that the CD4+/CD8+ thymocytes are the first to express virus and that this subset demonstrates the most rapid and extensive HIV-1-induced cell depletion. Following depletion of this subset, subsequent virus expression occurs predominantly in phenotypically CD4- cells, suggesting that CD4 down-regulation occurs in HIV-1-infected thymocytes in vivo. These results demonstrate the utility of these HIV-1 reporter constructs to monitor HIV pathogenesis in vitro and in vivo.

* Corresponding author. Mailing address: Departments of Medicine and Microbiology and Molecular Genetics, UCLA School of Medicine and UCLA AIDS Institute, 11-934 Factor Bldg., Box 951678, Los Angeles, CA 90095-1678. Phone: (310) 794-7765. Fax: (310) 825-6192. E-mail: jzack{at}ucla.edu.

J Virol, August 1998, p. 6520-6526, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Muthumani, K., Choo, A. Y., Shedlock, D. J., Laddy, D. J., Sundaram, S. G., Hirao, L., Wu, L., Thieu, K. P., Chung, C. W., Lankaraman, K. M., Tebas, P., Silvestri, G., Weiner, D. B. (2008). Human Immunodeficiency Virus Type 1 Nef Induces Programmed Death 1 Expression through a p38 Mitogen-Activated Protein Kinase-Dependent Mechanism. J. Virol. 82: 11536-11544 [Abstract] [Full Text]  
  • Roth, M. D., Whittaker, K. M., Choi, R., Tashkin, D. P., Baldwin, G. C. (2005). Cocaine and {sigma}-1 receptors modulate HIV infection, chemokine receptors, and the HPA axis in the huPBL-SCID model. J. Leukoc. Biol. 78: 1198-1203 [Abstract] [Full Text]  
  • Clay, C. C., Rodrigues, D. S., Harvey, D. J., Leutenegger, C. M., Esser, U. (2005). Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIVmac251-Infected and Uninfected Macaques. J. Virol. 79: 13759-13768 [Abstract] [Full Text]  
  • Choi, J., Walker, J., Talbert-Slagle, K., Wright, P., Pober, J. S., Alexander, L. (2005). Endothelial Cells Promote Human Immunodeficiency Virus Replication in Nondividing Memory T Cells via Nef-, Vpr-, and T-Cell Receptor-Dependent Activation of NFAT. J. Virol. 79: 11194-11204 [Abstract] [Full Text]  
  • Brown, A., Gartner, S., Kawano, T., Benoit, N., Cheng-Mayer, C. (2005). HLA-A2 down-regulation on primary human macrophages infected with an M-tropic EGFP-tagged HIV-1 reporter virus. J. Leukoc. Biol. 78: 675-685 [Abstract] [Full Text]  
  • Choudhary, S. K., Choudhary, N. R., Kimbrell, K. C., Colasanti, J., Ziogas, A., Kwa, D., Schuitemaker, H., Camerini, D. (2005). R5 Human Immunodeficiency Virus Type 1 Infection of Fetal Thymic Organ Culture Induces Cytokine and CCR5 Expression. J. Virol. 79: 458-471 [Abstract] [Full Text]  
  • Koka, P. S., Kitchen, C. M. R., Reddy, S. T. (2004). Targeting c-Mpl for Revival of Human Immunodeficiency Virus Type 1-Induced Hematopoietic Inhibition When CD34+ Progenitor Cells Are Re-Engrafted into a Fresh Stromal Microenvironment In Vivo. J. Virol. 78: 11385-11392 [Abstract] [Full Text]  
  • Bahbouhi, B., Landay, A., Al-Harthi, L. (2004). Dynamics of cytokine expression in HIV productively infected primary CD4+ T cells. Blood 103: 4581-4587 [Abstract] [Full Text]  
  • Ali, A., Lubong, R., Ng, H., Brooks, D. G., Zack, J. A., Yang, O. O. (2004). Impacts of Epitope Expression Kinetics and Class I Downregulation on the Antiviral Activity of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes. J. Virol. 78: 561-567 [Abstract] [Full Text]  
  • Brooks, D. G., Arlen, P. A., Gao, L., Kitchen, C. M. R., Zack, J. A. (2003). Identification of T cell-signaling pathways that stimulate latent HIV in primary cells. Proc. Natl. Acad. Sci. USA 100: 12955-12960 [Abstract] [Full Text]  
  • Ali, A., Pillai, S., Ng, H., Lubong, R., Richman, D. D., Jamieson, B. D., Ding, Y., McElrath, M. J., Guatelli, J. C., Yang, O. O. (2003). Broadly Increased Sensitivity to Cytotoxic T Lymphocytes Resulting from Nef Epitope Escape Mutations. J. Immunol. 171: 3999-4005 [Abstract] [Full Text]  
  • Qin, X.-F., An, D. S., Chen, I. S. Y., Baltimore, D. (2003). Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc. Natl. Acad. Sci. USA 100: 183-188 [Abstract] [Full Text]  
  • Scripture-Adams, D. D., Brooks, D. G., Korin, Y. D., Zack, J. A. (2002). Interleukin-7 Induces Expression of Latent Human Immunodeficiency Virus Type 1 with Minimal Effects on T-Cell Phenotype. J. Virol. 76: 13077-13082 [Abstract] [Full Text]  
  • Korin, Y. D., Brooks, D. G., Brown, S., Korotzer, A., Zack, J. A. (2002). Effects of Prostratin on T-Cell Activation and Human Immunodeficiency Virus Latency. J. Virol. 76: 8118-8123 [Abstract] [Full Text]  
  • Lundquist, C. A., Tobiume, M., Zhou, J., Unutmaz, D., Aiken, C. (2002). Nef-Mediated Downregulation of CD4 Enhances Human Immunodeficiency Virus Type 1 Replication in Primary T Lymphocytes. J. Virol. 76: 4625-4633 [Abstract] [Full Text]  
  • Brooks, D. G., Zack, J. A. (2002). Effect of Latent Human Immunodeficiency Virus Infection on Cell Surface Phenotype. J. Virol. 76: 1673-1681 [Abstract] [Full Text]  
  • Cole, S. W., Naliboff, B. D., Kemeny, M. E., Griswold, M. P., Fahey, J. L., Zack, J. A. (2001). Impaired response to HAART in HIV-infected individuals with high autonomic nervous system activity. Proc. Natl. Acad. Sci. USA 98: 12695-12700 [Abstract] [Full Text]  
  • Poles, M. A., Elliott, J., Taing, P., Anton, P. A., Chen, I. S. Y. (2001). A Preponderance of CCR5+ CXCR4+ Mononuclear Cells Enhances Gastrointestinal Mucosal Susceptibility to Human Immunodeficiency Virus Type 1 Infection. J. Virol. 75: 8390-8399 [Abstract] [Full Text]  
  • Logg, C. R., Logg, A., Tai, C.-K., Cannon, P. M., Kasahara, N. (2001). Genomic Stability of Murine Leukemia Viruses Containing Insertions at the Env-3' Untranslated Region Boundary. J. Virol. 75: 6989-6998 [Abstract] [Full Text]  
  • Zhu, Y., Gelbard, H. A., Roshal, M., Pursell, S., Jamieson, B. D., Planelles, V. (2001). Comparison of Cell Cycle Arrest, Transactivation, and Apoptosis Induced by the Simian Immunodeficiency Virus SIVagm and Human Immunodeficiency Virus Type 1 vpr Genes. J. Virol. 75: 3791-3801 [Abstract] [Full Text]  
  • Duus, K. M., Miller, E. D., Smith, J. A., Kovalev, G. I., Su, L. (2001). Separation of Human Immunodeficiency Virus Type 1 Replication from nef-Mediated Pathogenesis in the Human Thymus. J. Virol. 75: 3916-3924 [Abstract] [Full Text]  
  • Steinberger, P., Andris-Widhopf, J., Buhler, B., Torbett, B. E., Barbas, C. F. III (2000). Functional deletion of the CCR5 receptor by intracellular immunization produces cells that are refractory to CCR5-dependent HIV-1 infection and cell fusion. Proc. Natl. Acad. Sci. USA 97: 805-810 [Abstract] [Full Text]  
  • Koka, P. S., Jamieson, B. D., Brooks, D. G., Zack, J. A. (1999). Human Immunodeficiency Virus Type 1-Induced Hematopoietic Inhibition Is Independent of Productive Infection of Progenitor Cells In Vivo. J. Virol. 73: 9089-9097 [Abstract] [Full Text]  
  • Verhasselt, B., Naessens, E., Verhofstede, C., De Smedt, M., Schollen, S., Kerre, T., Vanhecke, D., Plum, J. (1999). Human Immunodeficiency Virus nef Gene Expression Affects Generation and Function of Human T Cells, But Not Dendritic Cells. Blood 94: 2809-2818 [Abstract] [Full Text]  
  • Chêne, L., Nugeyre, M.-T., Guillemard, E., Moulian, N., Barré-Sinoussi, F., Israël, N. (1999). Thymocyte-Thymic Epithelial Cell Interaction Leads to High-Level Replication of Human Immunodeficiency Virus Exclusively in Mature CD4+ CD8- CD3+ Thymocytes: a Critical Role for Tumor Necrosis Factor and Interleukin-7. J. Virol. 73: 7533-7542 [Abstract] [Full Text]  
  • An, D. S., Morizono, K., Li, Q.-X., Mao, S. H., Lu, S., Chen, I. S. Y. (1999). An Inducible Human Immunodeficiency Virus Type 1 (HIV-1) Vector Which Effectively Suppresses HIV-1 Replication. J. Virol. 73: 7671-7677 [Abstract] [Full Text]  
  • Amado, R. G., Jamieson, B. D., Cortado, R., Cole, S. W., Zack, J. A. (1999). Reconstitution of Human Thymic Implants Is Limited by Human Immunodeficiency Virus Breakthrough during Antiretroviral Therapy. J. Virol. 73: 6361-6369 [Abstract] [Full Text]  
  • Korin, Y. D., Zack, J. A. (1999). Nonproductive Human Immunodeficiency Virus Type 1 Infection in Nucleoside-Treated G0 Lymphocytes. J. Virol. 73: 6526-6532 [Abstract] [Full Text]  
  • Cole, S. W., Jamieson, B. D., Zack, J. A. (1999). cAMP Up-Regulates Cell Surface Expression of Lymphocyte CXCR4: Implications for Chemotaxis and HIV-1 Infection. J. Immunol. 162: 1392-1400 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»