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J Virol, August 1998, p. 6442-6447, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Biochemical Analysis of the Secreted and Virion Glycoproteins of Ebola Virus

Anthony Sanchez,1,* Zhi-Yong Yang,2 Ling Xu,2 Gary J. Nabel,2 Tamara Crews,3 and Clarence J. Peters1

Special Pathogens Branch, Division of Viral and Rickettsial Diseases,1 and Biotechnology Core Facility, Scientific Resources Program,3 National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, and Departments of Internal Medicine and Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109-06502

Received 12 January 1998/Accepted 17 April 1998

The glycoproteins expressed by a Zaire species of Ebola virus were analyzed for cleavage, oligomerization, and other structural properties to better define their functions. The 50- to 70-kDa secreted and 150-kDa virion/structural glycoproteins (SGP and GP, respectively), which share the 295 N-terminal residues, are cleaved near the N terminus by signalase. A second cleavage event, occurring in GP at a multibasic site (RRTRRdown-arrow ) that is likely mediated by furin, results in two glycoproteins (GP1 and GP2) linked by disulfide bonding. This furin cleavage site is present in the same position in the GPs of all Ebola viruses (R[R/K]X[R/K]Rdown-arrow ), and one is predicted for Marburg viruses (R[R/K]KRdown-arrow ), although in a different location. Based on the results of cross-linking studies, we were able to determine that Ebola virion peplomers are composed of trimers of GP1-GP2 heterodimers and that aspects of their structure are similar to those of retroviruses, paramyxoviruses, and influenza viruses. We also determined that SGP is secreted from infected cells almost exclusively in the form of a homodimer that is joined by disulfide bonding.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., Building 15, Room SB611, Mail Stop G14, Atlanta, GA 30333. Phone: (404) 639-1119. Fax: (404) 639-1118. E-mail: ans1{at}cdc.gov.

J Virol, August 1998, p. 6442-6447, Vol. 72, No. 8
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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