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J Virol, July 1998, p. 6260-6263, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Coreceptor Usage of Human Immunodeficiency Virus Type 2 Primary Isolates and Biological Clones Is Broad and Does Not Correlate with Their Syncytium-Inducing Capacities

Christophe Guillon,1 Marchina E. van der Ende,1,2 Patrick H. M. Boers,1 Rob A. Gruters,1,3 Martin Schutten,1 and Albert D. M. E. Osterhaus1,*

Institute of Virology, Erasmus University Rotterdam,1 and Department of Internal Medicine, University Hospital Dijkzigt,2 Rotterdam, The Netherlands, and UMR 103 CNRS/bioMérieux, Ecole Normale Supérieure de Lyon, Lyon, France3

Received 9 February 1998/Accepted 15 April 1998

Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by binding of the surface envelope glycoprotein to the CD4 molecule. Interaction of the resulting CD4-glycoprotein complex with alpha - or beta -chemokine receptors, depending on the biological phenotype of the virus, then initiates the fusion process. Here, we show that primary HIV-2 isolates and biological clones, in contrast to those of HIV-1, may use a broad range of coreceptors, including CCR-1, CCR-3, CCR-5, and CXCR-4. The syncytium-inducing capacity of these viruses did not correlate with the ability to infect via CXCR-4 or any other coreceptor. One cell-free passage of the intermediate isolates in mitogen-stimulated, CD8+ cell-depleted peripheral blood mononuclear cells resulted in the outgrowth of variants with CCR-5 only, whereas the coreceptor usage of late and early isolates did not change. Since HIV-2 is less pathogenic in vivo than HIV-1, these data suggest that HIV pathogenicity in vivo is not directly related to the spectrum of coreceptors used in in vitro systems.

* Corresponding author. Mailing address: Institute of Virology, Erasmus University Rotterdam, Dr. Molewaterplein 50, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands. Phone: (31) 10 408 8066. Fax: (31) 10 436 5145. E-mail: osterhaus{at}viro.fgg.eur.nl

J Virol, July 1998, p. 6260-6263, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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