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J Virol, July 1998, p. 6257-6259, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Inhibition of Human Immunodeficiency Virus Type 1 Virion Entry by Dominant-Negative Hck

Kenzo Tokunaga,1,dagger Etsuko Kiyokawa,1 Mie Nakaya,2 Naomi Otsuka,2 Asato Kojima,1 Takeshi Kurata,1 and Michiyuki Matsuda1,2,*

Department of Pathology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640,1 and Department of Pathology, Research Institute, International Medical Center of Japan, Toyama, Shinjuku-ku, Tokyo 162-8655,2 Japan

Received 22 December 1997/Accepted 27 February 1998

To study the role of Src family tyrosine kinases in infection with human immunodeficiency virus type 1 (HIV-1), we constructed an Hck mutant, HckN, that hinders signaling from wild-type Hck. HIV-1 produced in HckN-expressing cells was significantly less infectious to HeLa-CD4-LTR-beta -gal (MAGI) cells than HIV-1 produced in mock-transfected cells. The inhibitory effect of HckN was compensated for by the expression of vesicular stomatitis virus G protein. Finally, we found that the HIV-1 produced in the HckN-expressing cells entered into the cells less efficiently than did the control HIV-1. These results suggest that the Src family tyrosine kinases regulate entry of HIV-1 into target cells.

* Corresponding author. Mailing address: Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655. Japan. Phone: 81-3-3202-7181. Fax: 81-3-3202-7364. E-mail address: mmatsuda @nih .go .jp.

dagger Present address. Unite de Biologie des Retrovirus, Departement de Virologie, Institut Pasteur, Paris, France.

J Virol, July 1998, p. 6257-6259, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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