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J Virol, July 1998, p. 6251-6256, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the Molecular Mechanism of Defective Interfering RNA-Mediated Symptom Attenuation in Tombusvirus-Infected Plants

Zoltán Havelda, György Szittya, and József Burgyán*

Agricultural Biotechnology Center, Plant Science Institute, 2101 Gödöllö, Hungary

Received 11 December 1997/Accepted 9 April 1998

Different tombusviruses were able to support the replication of either homologous or heterologous defective interfering (DI) RNAs, and those infected plants usually developed typical attenuated symptoms. However, in some helper virus-DI RNA combinations the inoculated plants were necrotized, although they contained a high level of DI RNA, suggesting that the accumulation of DI RNA and the resulting suppression of genomic RNA replication were not directly responsible for the symptom attenuation. Moreover, the 19-kDa protein product of ORF 5, which is known to play a crucial role in necrotic symptom development, accumulated at the same level in the infected plants in the presence of protective homologous DI RNA and in the presence of nonprotective heterologous DI RNA. It was also demonstrated, by chimeric helper viruses, that the ability of heterologous DI RNA to protect the virus-infected plants against systemic necrosis is determined by the 5'-proximal region of the helper virus genome. The results presented suggest that DI RNA-mediated protection did not operate via the specific inhibition of 19-kDa protein expression but, more likely, DI RNAs in protective DI-helper virus combinations specifically interacted with viral products, preventing the induction of necrotic symptoms.

* Corresponding author. Mailing address: Agricultural Biotechnology Center, Plant Science Institute, P.O. Box 411, 2101 Gödöllö, Hungary. Phone: (36-28)430-600. Fax: (36-28)430-482. E-mail: burgyan{at}abc.hu.

J Virol, July 1998, p. 6251-6256, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Havelda, Z., Hornyik, C., Valoczi, A., Burgyan, J. (2005). Defective Interfering RNA Hinders the Activity of a Tombusvirus-Encoded Posttranscriptional Gene Silencing Suppressor. J. Virol. 79: 450-457 [Abstract] [Full Text]  
  • Rubino, L., Pantaleo, V., Navarro, B., Russo, M. (2004). Expression of tombusvirus open reading frames 1 and 2 is sufficient for the replication of defective interfering, but not satellite, RNA. J. Gen. Virol. 85: 3115-3122 [Abstract] [Full Text]  
  • Havelda, Z., Hornyik, C., Crescenzi, A., Burgyan, J. (2003). In Situ Characterization of Cymbidium Ringspot Tombusvirus Infection-Induced Posttranscriptional Gene Silencing in Nicotiana benthamiana. J. Virol. 77: 6082-6086 [Abstract] [Full Text]  
  • Szittya, G., Molnar, A., Silhavy, D., Hornyik, C., Burgyan, J. (2002). Short Defective Interfering RNAs of Tombusviruses Are Not Targeted but Trigger Post-Transcriptional Gene Silencing against Their Helper Virus. Plant Cell 14: 359-372 [Abstract] [Full Text]  
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  • Burgyán, J., Hornyik, C., Szittya, G., Silhavy, D., Bisztray, G. (2000). The ORF1 Products of Tombusviruses Play a Crucial Role in Lethal Necrosis of Virus-Infected Plants. J. Virol. 74: 10873-10881 [Abstract] [Full Text]  


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