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J Virol, July 1998, p. 6223-6227, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Herpesvirus 8 in the Prostate Glands of Men with Kaposi's Sarcoma

Catherine Diamond,1,2 Scott J. Brodie,3 John N. Krieger,1,4 Meei-Li Huang,2 David M. Koelle,1,2,3 Kurt Diem,3 David Muthui,3 and Lawrence Corey1,2,3,*

Departments of Medicine,1 Laboratory Medicine,3 and Urology,4 University of Washington, and Program in Infectious Diseases, Fred Hutchinson Cancer Research Center,2 Seattle, Washington

Received 29 December 1997/Accepted 13 April 1998

The epidemiology of human immunodeficiency virus (HIV)-associated Kaposi's sarcoma (KS) resembles that of a sexually transmitted pathogen. However, human herpesvirus 8 (HHV-8), the proposed cause of KS, is found in semen only infrequently and at low titers. To determine whether HHV-8 was present in the urogenital tract, transrectal ultrasound-guided prostate biopsies were obtained from six men with KS (five with concurrent HIV infection) and four without KS (three with concurrent HIV) and assayed for HHV-8 by PCR. Nine of the 10 men were seropositive for HHV-8. Five of nine HHV-8-seropositive men had HHV-8 DNA detected in prostate tissue by solution-based PCR. All five currently had KS or had it previously. In two subjects, prostate tissue was the only identified source of HHV-8. In situ PCR on serial sections of prostate indicated that HHV-8 infection was localized to discrete areas of the prostate. When detected, HHV-8 DNA was present in the nuclei of >90% of the glandular epithelial cells. In situ hybridization for HHV-8 mRNA revealed that between 1 and 5% of cells harboring HHV-8 DNA expressed viral transcripts associated with HHV-8 replication (T1.1 transcript), while >90% expressed gene products associated with viral latency (T0.7 transcript). Intermittent replication of HHV-8 in the prostate and subsequent shedding of virus in semen may be crucial factors for determining whether HHV-8 can be transmitted through sexual activity.

* Corresponding author. Mailing address: 1124 Columbia St., M-115, Seattle, WA 98104. Phone: (206) 667-6702. Fax: (206) 667-4411. E-mail: lcorey{at}u.washington.edu.

J Virol, July 1998, p. 6223-6227, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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