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J Virol, July 1998, p. 6190-6194, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Minimal Avian Retroviral Packaging Sequence Has a Complex Structure

Jennifer D. Banks,1,2 Ashly Yeo,1 Kristen Green,1 Franzmarie Cepeda,1 and Maxine L. Linial1,2,*

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,1 and Department of Microbiology, University of Washington, Seattle, Washington 981952

Received 15 October 1997/Accepted 26 March 1998

We have defined a 160-nucleotide region, Mpsi , from the 5' leader region of the Rous sarcoma virus genome that is sufficient to direct the packaging of a heterologous RNA. Mpsi contains the putative O3 stem structure that has previously been shown, and that has been confirmed in this study, to be important for the efficient packaging of avian leukosis-sarcoma virus RNA. Analyses of several O3 stem mutants revealed that other regions within Mpsi can interfere with the proper folding of altered sequences which are predicted to form a wild-type O3 stem.

* Corresponding author. Mailing address: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024. Phone: (206) 667-4442. Fax: (206) 667-5939. E-mail: mlinial{at}fred.fhcrc.org.

J Virol, July 1998, p. 6190-6194, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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