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J Virol, July 1998, p. 6151-6154, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Human Papillomavirus Type 11 Recombinant L1
Capsomeres Induce Virus-Neutralizing Antibodies
Robert C.
Rose,1,2,*
Wendy I.
White,3
Maolin
Li,4
JoAnn A.
Suzich,3
Christopher
Lane,1 and
Robert L.
Garcea4
Departments of
Medicine1 and
Microbiology and
Immunology,2
University of Rochester
School of Medicine and Dentistry, Rochester, New York 14642; MedImmune,
Inc., Gaithersburg, Maryland 208783; and
Section of Pediatric Hematology/Oncology, University of
Colorado School of Medicine, Denver, Colorado 802624
Received 7 January 1998/Accepted 27 March 1998
The human papillomavirus type 11 (HPV-11) L1 major capsid protein
can be trypsinized to generate recombinant capsomeres that retain HPV
genotype-restricted capsid antigenicity (M. Li, T. P. Cripe,
P. A. Estes, M. K. Lyon, R. C. Rose, and R. L. Garcea, J. Virol. 71:2988-2995, 1997). In the present study,
HPV-11 virion-neutralizing monoclonal antibodies H11.F1 and H11.H3,
previously characterized as recognizing two distinct HPV-11
capsid-neutralizing antigenic domains (S. W. Ludmerer, D. Benincasa, and G. E. Mark III, J. Virol. 70:4791-4794,
1996), were each found to be highly immunoreactive with
trypsin-generated capsomeres in an enzyme-linked immunosorbent assay
(ELISA). Capsomeres were used to generate high-titer polyclonal immune
sera that demonstrated HPV genotype-restricted reactivity by ELISA. The
capsomere antisera were then tested in an in vitro infectivity assay
and found to neutralize HPV-11 virions. In this assay, HPV-11 capsomere
polyclonal antisera exhibited neutralization titers (10
5
to 10
6) comparable to those obtained with a
virion-neutralizing antiserum raised previously against intact HPV-11
VLPs (R. C. Rose, R. C. Reichman, and W. Bonnez, J. Gen.
Virol. 75:2075-2079, 1994). These results indicate that highly
immunogenic, genotype-restricted HPV capsid-neutralizing antigenic
domains are contained entirely within capsomeres. Thus, capsomeres may
be viable vaccine candidates for the prevention of HPV disease.
*
Corresponding author. Mailing address: University of
Rochester School of Medicine, Department of Medicine, Box 689, 601 Elmwood Ave., Rochester, NY 14642. Phone: (716) 275-5871. Fax: (716)
442-9328. E-mail: rrose{at}medicine.rochester.edu.
J Virol, July 1998, p. 6151-6154, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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