Previous Article | Next Article ![]()
J Virol, July 1998, p. 5781-5788, Vol. 72, No. 7
Department of Biochemistry and Howard Hughes
Medical Institute, Stanford University Medical Center, Stanford,
California 94305,1 and
SyStemix
Incorporated, Palo Alto, California 943042
Received 7 October 1997/Accepted 30 March 1998
Lentiviruses are potentially advantageous compared to
oncoretroviruses as gene transfer agents because they can infect
nondividing cells. We demonstrate here that human immunodeficiency
virus type 1 (HIV-1)-based vectors were highly efficient in transducing
purified human hematopoietic stem cells. Transduction rates, measured
by marker gene expression or by PCR of the integrated provirus,
exceeded 50%, and transduction appeared to be independent of mitosis.
Derivatives of HIV-1 were constructed to optimize the vector, and a
deletion of most of Vif and Vpr was required to ensure the long-term
persistence of transduced cells with relatively stable expression of
the marker gene product. These results extend the utility of this
lentivirus vector system.
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Human Immunodeficiency Virus Type 1 Vectors
Efficiently Transduce Human Hematopoietic Stem Cells
*
Corresponding author. Mailing address: 253 Beckman
Center, Stanford University Medical Center, Stanford, CA 94305. Phone: (650) 725-7569. Fax: (650) 723-1399. E-mail:
sutton{at}cmgm.stanford.edu.
This article has been cited by other articles:
| J. Bacteriol. | Mol. Cell. Biol. | Microbiol. Mol. Biol. Rev. |
|---|
| Clin. Vaccine Immunol. | ALL ASM JOURNALS |
|---|