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J Virol, July 1998, p. 5781-5788, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells

Richard E. Sutton,1,* Henry T. M. Wu,1 Richard Rigg,2 Ernst Böhnlein,2 and Patrick O. Brown1

Department of Biochemistry and Howard Hughes Medical Institute, Stanford University Medical Center, Stanford, California 94305,1 and SyStemix Incorporated, Palo Alto, California 943042

Received 7 October 1997/Accepted 30 March 1998

Lentiviruses are potentially advantageous compared to oncoretroviruses as gene transfer agents because they can infect nondividing cells. We demonstrate here that human immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing purified human hematopoietic stem cells. Transduction rates, measured by marker gene expression or by PCR of the integrated provirus, exceeded 50%, and transduction appeared to be independent of mitosis. Derivatives of HIV-1 were constructed to optimize the vector, and a deletion of most of Vif and Vpr was required to ensure the long-term persistence of transduced cells with relatively stable expression of the marker gene product. These results extend the utility of this lentivirus vector system.

* Corresponding author. Mailing address: 253 Beckman Center, Stanford University Medical Center, Stanford, CA 94305. Phone: (650) 725-7569. Fax: (650) 723-1399. E-mail: sutton{at}cmgm.stanford.edu.

J Virol, July 1998, p. 5781-5788, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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