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J Virol, July 1998, p. 5457-5463, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the Transcriptional Repressive Element of the Human Cytomegalovirus Immediate-Early US3 Gene

Bonita J. Biegalke*

Department of Biological Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701

Received 20 February 1998/Accepted 7 April 1998

Transcriptional repression is utilized by human cytomegalovirus to regulate expression of the immediate-early US3 gene. Sequences located 3' of the US3 TATA box are required for down regulation of expression. Mutagenesis of US3 sequences identified a 10-nucleotide region that is essential for transcriptional repression. In addition to the 10-nucleotide element, an additional region, which includes the US3 initiator element, was needed to confer repression on a heterologous promoter. Thus, a 19-nucleotide element (-18 to +1 relative to the transcription start site) functioned as a transcriptional repressive element (tre). The tre repressed transcription in a position-dependent but orientation-independent manner. In vivo footprinting experiments demonstrated that transcriptional repression is associated with a decrease in protein interactions with the US3 promoter and surrounding sequences. The data presented here suggest that the association of an as yet unidentified repressor protein with the tre represses transcription by inhibiting assembly of the transcription initiation complex on the US3 promoter.

* Mailing address: Department of Biological Sciences, 108 Irvine Hall, Ohio University, Athens, OH 45701. Phone: (740) 593-2377. Fax: (740) 593-0300. E-mail: biegalke{at}ohiou.edu.

J Virol, July 1998, p. 5457-5463, Vol. 72, No. 7
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Sengupta, P. K., Fargo, J., Smith, B. D. (2002). The RFX Family Interacts at the Collagen (COL1A2) Start Site and Represses Transcription. J. Biol. Chem. 277: 24926-24937 [Abstract] [Full Text]  
  • LaPierre, L. A., Biegalke, B. J. (2001). Identification of a Novel Transcriptional Repressor Encoded by Human Cytomegalovirus. J. Virol. 75: 6062-6069 [Abstract] [Full Text]  
  • Lashmit, P. E., Stinski, M. F., Murphy, E. A., Bullock, G. C. (1998). A cis Repression Sequence Adjacent to the Transcription Start Site of the Human Cytomegalovirus US3 Gene Is Required To Down Regulate Gene Expression at Early and Late Times after Infection. J. Virol. 72: 9575-9584 [Abstract] [Full Text]  


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