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J Virol, June 1998, p. 5318-5322, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene

Christian Moser, Jon-Duri Tratschin,* and Martin A. Hofmann

Institute of Virology and Immunoprophylaxis, CH-3147 Mittelhäusern, Switzerland

Received 20 November 1997/Accepted 2 March 1998

The gene coding for bacterial chloramphenicol acetyltransferase (CAT) was inserted in frame into the viral Npro gene of the full-length cDNA clone pA187-1 of the classical swine fever virus (CSFV) strain Alfort/187. RNA transcribed in vitro from the resulting plasmid was transfected into SK-6 porcine kidney cells. Infectious progeny virus vA187-CAT recovered from transfected cells had growth characteristics indistinguishable from those of parental virus vA187-1. In cells infected with vA187-CAT the predicted fusion protein, CAT-Npro, was detected, and it retained the enzymatic activities of both CAT and Npro. The CAT gene remained stably inserted in the viral genome after 10 virus passages. Thus, marker virus vA187-CAT represents a useful tool for quantitative analysis of viral replication and gene expression.

* Corresponding author. Mailing address: Institute of Virology and Immunoprophylaxis, CH-3147 Mittelhäusern, Switzerland. Phone: 41 31 848 92 11. Fax: 41 31 848 92 22. E-mail: jon-duri.tratschin{at}ivi.admin.ch.

J Virol, June 1998, p. 5318-5322, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.