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J Virol, June 1998, p. 5271-5275, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Immunodeficiency Virus Type 2 Vpx-Gag Interaction

Heather A. Pancio and Lee Ratner*

Departments of Medicine, Pathology, and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110

Received 12 August 1996/Accepted 9 February 1998

Incorporation of Vpx into human immunodeficiency virus type 2 (HIV-2) virus-like particles is mediated by the Gag polyprotein. We have identified residues 15 to 40 of Gag p6 and residues 73 to 89 of Vpx as being necessary for virion incorporation. In addition, we show enhanced in vitro binding of Vpx to a chimeric HIV-1/HIV-2 Gag construct containing residues 2 to 49 of HIV-2 p6 and demonstrate that the presence of residues 73 to 89 of Vpx allows for in vitro binding to HIV-2 Gag.

* Corresponding author. Mailing address: Box 8069, 660 S. Euclid, Washington University School of Medicine, St. Louis, MO 63110. Phone: (314) 362-8836. Fax: (314) 454-1792. E-mail: lratner{at}imgate.wustl.edu.

J Virol, June 1998, p. 5271-5275, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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