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J Virol, June 1998, p. 4950-4955, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
L* Protein of the DA Strain of Theiler's Murine
Encephalomyelitis Virus Is Important for Virus Growth in a Murine
Macrophage-Like Cell Line
Hisashi
Takata,1
Masatsugu
Obuchi,2
Jiro
Yamamoto,1
Takato
Odagiri,2
Raymond P.
Roos,3
Hideaki
Iizuka,1 and
Yoshiro
Ohara2,*
Departments of
Neurosurgery1 and
Microbiology,2 Kanazawa Medical
University, Uchinada, Ishikawa 920-0293, Japan, and
Department of Neurology, University of Chicago, Chicago,
Illinois 606373
Received 23 October 1997/Accepted 22 February 1998
Strain GDVII and other members of the GDVII subgroup of Theiler's
murine encephalomyelitis virus (TMEV) are highly virulent and cause
acute polioencephalomyelitis in mice. Neither viral persistence nor
demyelination is demonstrated in the few surviving mice. On the other
hand, strain DA and other members of the TO subgroup of TMEV are less
virulent and establish a persistent infection in the spinal cord, which
results in a demyelinating disease. We previously reported that GDVII
does not actively replicate in a murine macrophage-like cell line,
J774-1, whereas DA strain productively infects these cells (M. Obuchi,
Y. Ohara, T. Takegami, T. Murayama, H. Takada, and H. Iizuka, J. Virol. 71:729-733, 1997). In the present study, we used recombinant
viruses between these strains of the two subgroups to demonstrate that
the DA L coding region of DA strain is important for virus growth in
J774-1 cells. Additional experiments with a mutant virus indicate that
L* protein, which is synthesized out of frame with the polyprotein from
an additional alternative initiation codon in the L coding region of TO
subgroup strains, is a key determinant responsible for the cell-type-specific restriction of virus growth. L* protein may play a
critical role in the DA-induced restricted demyelinating infection by
allowing growth in macrophages, a major site for virus persistence.
*
Corresponding author. Mailing address: Department of
Microbiology, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan. Phone: 81-76-286-2211. Fax: 81-76-286-3961. E-mail: ohara{at}kanazawa-med.ac.jp.
J Virol, June 1998, p. 4950-4955, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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