This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eleouet, J.-F.
Right arrow Articles by Laude, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eleouet, J.-F.
Right arrow Articles by Laude, H.

 Previous Article  |  Next Article 

J Virol, June 1998, p. 4918-4924, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transmissible Gastroenteritis Coronavirus Induces Programmed Cell Death in Infected Cells through a Caspase-Dependent Pathway

Jean-François Eleouet,* Stefan Chilmonczyk, Lydia Besnardeau, and Hubert Laude

Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France

Received 15 October 1997/Accepted 16 March 1998

In this report, we show that apoptosis (or programmed cell death) is induced in different cell lines infected with a coronavirus, the porcine transmissible gastroenteritis virus (TGEV). Kinetic analysis of internucleosomal DNA cleavage by agarose gel electrophoresis and flow cytometry or cytometric monitoring of the mitochondrial transmembrane potential showed that, for ST cells infected with TGEV, the first overt signs of apoptosis appeared from 10 to 12 h postinfection on. They preceded morphological changes characteristic of cells undergoing apoptosis, as observed by light and electron microscopy. The tripeptide pan-ICE (caspase) inhibitor N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone blocked TGEV-induced apoptosis with no effect on virus production. The thiol agent pyrrolidine dithiocarbamate inhibited apoptosis, suggesting that TGEV infection may lead to apoptosis via cellular oxidative stress. The effect of TGEV infection on activation of NF-kappa B, a transcription factor known to be activated by oxidative stress, was examined. NF-kappa B DNA binding was shown to be strongly and quickly induced by TGEV infection. However, transcription factor decoy experiments showed that NF-kappa B activation is not critical for TGEV-induced apoptosis.

* Corresponding author. Mailing address: INRA, Unité de Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas cedex, France. Phone: (33) 1 34 65 26 41. Fax: (33) 1 34 65 26 21. E-mail: eleouet{at}biotec.jouy.inra.fr.

J Virol, June 1998, p. 4918-4924, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yang, C.-W., Yang, Y.-N., Liang, P.-H., Chen, C.-M., Chen, W.-L., Chang, H.-Y., Chao, Y.-S., Lee, S.-J. (2007). Novel Small-Molecule Inhibitors of Transmissible Gastroenteritis Virus. Antimicrob. Agents Chemother. 51: 3924-3931 [Abstract] [Full Text]  
  • Fennewald, S. M., Scott, E. P., Zhang, L., Yang, X., Aronson, J. F., Gorenstein, D. G., Luxon, B. A., Shope, R. E., Beasley, D. W. C., Barrett, A. D. T., Herzog, N. K. (2007). Thioaptamer decoy targeting of AP-1 proteins influences cytokine expression and the outcome of arenavirus infections. J. Gen. Virol. 88: 981-990 [Abstract] [Full Text]  
  • Liu, M., Vakharia, V. N. (2006). Nonstructural protein of infectious bursal disease virus inhibits apoptosis at the early stage of virus infection.. J. Virol. 80: 3369-3377 [Abstract] [Full Text]  
  • Natoni, A., Kass, G. E. N., Carter, M. J., Roberts, L. O. (2006). The mitochondrial pathway of apoptosis is triggered during feline calicivirus infection. J. Gen. Virol. 87: 357-361 [Abstract] [Full Text]  
  • Tan, Y.-J., Fielding, B. C., Goh, P.-Y., Shen, S., Tan, T. H. P., Lim, S. G., Hong, W. (2004). Overexpression of 7a, a Protein Specifically Encoded by the Severe Acute Respiratory Syndrome Coronavirus, Induces Apoptosis via a Caspase-Dependent Pathway. J. Virol. 78: 14043-14047 [Abstract] [Full Text]  
  • Ortego, J., Escors, D., Laude, H., Enjuanes, L. (2002). Generation of a Replication-Competent, Propagation-Deficient Virus Vector Based on the Transmissible Gastroenteritis Coronavirus Genome. J. Virol. 76: 11518-11529 [Abstract] [Full Text]  
  • Chen, H., Wurm, T., Britton, P., Brooks, G., Hiscox, J. A. (2002). Interaction of the Coronavirus Nucleoprotein with Nucleolar Antigens and the Host Cell. J. Virol. 76: 5233-5250 [Abstract] [Full Text]  
  • Liu, C., Xu, H. Y., Liu, D. X. (2001). Induction of Caspase-Dependent Apoptosis in Cultured Cells by the Avian Coronavirus Infectious Bronchitis Virus. J. Virol. 75: 6402-6409 [Abstract] [Full Text]  
  • Banerjee, S., An, S., Zhou, A., Silverman, R. H., Makino, S. (2000). RNase L-Independent Specific 28S rRNA Cleavage in Murine Coronavirus-Infected Cells. J. Virol. 74: 8793-8802 [Abstract] [Full Text]  
  • Eléouët, J.-F., Slee, E. A., Saurini, F., Castagné, N., Poncet, D., Garrido, C., Solary, E., Martin, S. J. (2000). The Viral Nucleocapsid Protein of Transmissible Gastroenteritis Coronavirus (TGEV) Is Cleaved by Caspase-6 and -7 during TGEV-Induced Apoptosis. J. Virol. 74: 3975-3983 [Abstract] [Full Text]  
  • An, S., Chen, C.-J., Yu, X., Leibowitz, J. L., Makino, S. (1999). Induction of Apoptosis in Murine Coronavirus-Infected Cultured Cells and Demonstration of E Protein as an Apoptosis Inducer. J. Virol. 73: 7853-7859 [Abstract] [Full Text]  
  • Lin, K.-I, Pasinelli, P., Brown, R. H., Hardwick, J. M., Ratan, R. R. (1999). Decreased Intracellular Superoxide Levels Activate Sindbis Virus-induced Apoptosis. J. Biol. Chem. 274: 13650-13655 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»