This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Porter, C. D.
Right arrow Articles by Collins, M. K. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Porter, C. D.
Right arrow Articles by Collins, M. K. L.

 Previous Article  |  Next Article 

J Virol, June 1998, p. 4832-4840, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cationic Liposomes Enhance the Rate of Transduction by a Recombinant Retroviral Vector In Vitro and In Vivo

Colin D. Porter,1,* Katalin V. Lukacs,1,2 Gary Box,1 Yasuhiro Takeuchi,1 and Mary K. L. Collins1,dagger

Chester Beatty Laboratories, Institute of Cancer Research, London SW3 6JB,1 and National Heart and Lung Institute, Imperial College, London SW3 6LR,2 United Kingdom

Received 15 October 1997/Accepted 20 February 1998

Cationic liposomes enhanced the rate of transduction of target cells with retroviral vectors. The greatest effect was seen with the formulation DC-Chol/DOPE, which gave a 20-fold increase in initial transduction rate. This allowed an efficiency of transduction after brief exposure of target cells to virus plus liposome that could be achieved only after extensive exposure to virus alone. Enhancement with DC-Chol/DOPE was optimal when stable virion-liposome complexes were preformed. The transduction rate for complexed virus, as for virus used alone or with the polycation Polybrene, showed first-order dependence on virus concentration. Cationic liposomes, but not Polybrene, were able to mediate envelope-independent transduction, but optimal efficiency required envelope-receptor interaction. When virus complexed with DC-Chol/DOPE was used to transduce human mesothelioma xenografts, transduction was enhanced four- to fivefold compared to that for virus alone. Since the efficacy of gene therapy is dependent on the number of cells modified, which is in turn dependent upon the balance between transduction and biological clearance of the vector, the ability of cationic liposomes to form stable complexes with retroviral vectors and enhance their rate of infection is likely to be important for in vivo application.

* Corresponding author. Mailing address: Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Rd., London SW3 6JB, United Kingdom. Phone: 171 352 8133. Fax: 171 352 3299. E-mail: c.porter{at}icr.ac.uk.

dagger Present address: Department of Immunology, Windeyer Institute of Medical Science, London W1P 6DB, United Kingdom.

J Virol, June 1998, p. 4832-4840, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sanders, R. W., Venturi, M., Schiffner, L., Kalyanaraman, R., Katinger, H., Lloyd, K. O., Kwong, P. D., Moore, J. P. (2002). The Mannose-Dependent Epitope for Neutralizing Antibody 2G12 on Human Immunodeficiency Virus Type 1 Glycoprotein gp120. J. Virol. 76: 7293-7305 [Abstract] [Full Text]  
  • McKnight, A., Griffiths, D. J., Dittmar, M., Clapham, P., Thomas, E. (2001). Characterization of a Late Entry Event in the Replication Cycle of Human Immunodeficiency Virus Type 2. J. Virol. 75: 6914-6922 [Abstract] [Full Text]  
  • Specke, V., Tacke, S. J., Boller, K., Schwendemann, J., Denner, J. (2001). Porcine endogenous retroviruses: in vitro host range and attempts to establish small animal models. J. Gen. Virol. 82: 837-844 [Abstract] [Full Text]  
  • Trkola, A., Matthews, J., Gordon, C., Ketas, T., Moore, J. P. (1999). A Cell Line-Based Neutralization Assay for Primary Human Immunodeficiency Virus Type 1 Isolates That Use either the CCR5 or the CXCR4 Coreceptor. J. Virol. 73: 8966-8974 [Abstract] [Full Text]  
  • Trkola, A., Gordon, C., Matthews, J., Maxwell, E., Ketas, T., Czaplewski, L., Proudfoot, A. E. I., Moore, J. P. (1999). The CC-Chemokine RANTES Increases the Attachment of Human Immunodeficiency Virus Type 1 to Target Cells via Glycosaminoglycans and Also Activates a Signal Transduction Pathway That Enhances Viral Infectivity. J. Virol. 73: 6370-6379 [Abstract] [Full Text]  
  • Martin, F., Neil, S., Kupsch, J., Maurice, M., Cosset, F.-L., Collins, M. (1999). Retrovirus Targeting by Tropism Restriction to Melanoma Cells. J. Virol. 73: 6923-6929 [Abstract] [Full Text]  
  • Gordon, C. J., Muesing, M. A., Proudfoot, A. E. I., Power, C. A., Moore, J. P., Trkola, A. (1999). Enhancement of Human Immunodeficiency Virus Type 1 Infection by the CC-Chemokine RANTES Is Independent of the Mechanism of Virus-Cell Fusion. J. Virol. 73: 684-694 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»