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J Virol, June 1998, p. 4756-4764, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cell-Specific Expression of RANTES, MCP-1, and MIP-1alpha by Lower Airway Epithelial Cells and Eosinophils Infected with Respiratory Syncytial Virus

Barbara Olszewska-Pazdrak,1 Antonella Casola,1 Tadahito Saito,1 Rafeul Alam,2 Sheila E. Crowe,2 Fang Mei,1 Pearay L. Ogra,1 and Roberto P. Garofalo1,*

Departments of Pediatrics1 and Internal Medicine,2 University of Texas Medical Branch, Galveston, Texas 77555-0369

Received 15 January 1998/Accepted 2 March 1998

Respiratory syncytial virus (RSV) is the major cause of acute bronchiolitis in infancy, a syndrome characterized by wheezing, respiratory distress, and the pathologic findings of peribronchial mononuclear cell infiltration and release of inflammatory mediators by basophil and eosinophil leukocytes. Composition and activation of this cellular response are thought to rely on the discrete target cell selectivity of C-C chemokines. We demonstrate that infection in vitro of human epithelial cells of the lower respiratory tract by RSV induced dose- and time-dependent increases in mRNA and protein secretion for RANTES (regulated upon activation, normal T-cell expressed and presumably secreted), monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-1alpha (MIP-1alpha ). Production of MCP-1 and MIP-1alpha was selectively localized only in epithelial cells of the small airways and lung. Exposure of epithelial cells to gamma interferon (IFN-gamma ), in combination with RSV infection, induced a significant increase in RANTES production that was synergistic with respect to that obtained by RSV infection or IFN-gamma treatment alone. Epithelial cell-derived chemokines exhibited a strong chemotactic activity for normal human blood eosinophils. Furthermore, eosinophils were susceptible to RSV and released RANTES and MIP-1alpha as a result of infection. Therefore, the inflammatory process in RSV-induced bronchiolitis appears to be triggered by the infection of epithelial cells and further amplified via mechanisms driven by IFN-gamma and by the secretion of eosinophil chemokines.

* Corresponding author. Mailing address: Division of Immunology/Allergy/Rheumatology, 301 University Blvd., Galveston, TX 77555-0369. Phone: (409) 772-2298. Fax: (409) 772-1761. E-mail: Roberto.Garofalo{at}UTMB.edu.

J Virol, June 1998, p. 4756-4764, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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