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J Virol, June 1998, p. 4552-4559, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Characterization of Determinants for Envelope
Binding and Infection in Tva, the Subgroup A Avian Sarcoma and
Leukosis Virus Receptor
Lijun
Rong,
Kristin
Gendron,
Barbara
Strohl,
Rajeev
Shenoy,
Rouven J.
Wool-Lewis, and
Paul
Bates*
Department of Microbiology, School of
Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
Received 1 December 1997/Accepted 11 February 1998
Tva is the cellular receptor for subgroup A avian leukosis and
sarcoma virus (ALSV-A). The viral interaction domain of Tva is
determined by a 40-residue, cysteine-rich module closely related to the
ligand binding domain of the human low-density lipoprotein receptor
(LDLR). In this report, we examined the role of the LDLR-like module of
Tva in envelope binding and viral infection by mutational analysis. We
found that the entire LDLR module in Tva is essential for efficient
binding to the viral envelope protein. However, the 17 N-terminal
residues of this module can be deleted without affecting receptor
function, suggesting that the major determinants for viral entry are
located at the C terminus of the module. The effect on viral infection
of many amino acid substitutions and deletions in the LDLR module is
context dependent, suggesting that the residues important for viral
entry are dispersed throughout the LDLR module. In addition, we found
that all 27 mutations at residues D46, E47, and W48 greatly reduced
envelope binding. These results are discussed in relation to a recently
elucidated structure for an LDLR module.
*
Corresponding author. Mailing address: Department of
Microbiology, 225 Johnson Pavilion, 3610 Hamilton Walk, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076. Phone:
(215) 573-3509. Fax: (215) 573-4184. E-mail:
pbates{at}mail.med.upenn.edu.
J Virol, June 1998, p. 4552-4559, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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