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J Virol, May 1998, p. 4537-4540, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Evidence for Nosocomial Transmission of Human Immunodeficiency Virus from a Surgeon to One of His Patients

Alain Blanchard,* Stéphane Ferris, Sophie Chamaret, Denise Guétard, and Luc Montagnier

Unité d'Oncologie Virale, Institut Pasteur, 75724 Paris Cedex 15, France

Received 29 January 1997/Accepted 15 December 1997

We have investigated the molecular evidence in favor of the transmission of human immunodeficiency virus (HIV) from an HIV-infected surgeon to one of his patients. After PCR amplification, the env and gag sequences from the viral genome were cloned and sequenced. Phylogenetic analysis revealed that the viral sequences derived from the surgeon and his patient are closely related, which strongly suggests that nosocomial transmission occurred. In addition, these viral sequences belong to group M of HIV type 1 but are divergent from the reference sequences of the known subtypes.

* Corresponding author. Mailing address: Institut Pasteur, Unité d'Oncologie Virale, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 1-40-61-31-31. Fax: 1-40-61-34-65. E-mail: ablancha{at}pasteur.fr.

J Virol, May 1998, p. 4537-4540, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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