This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duan, X.
Right arrow Articles by Pensaert, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duan, X.
Right arrow Articles by Pensaert, M. B.

 Previous Article  |  Next Article 

J Virol, May 1998, p. 4520-4523, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of a Putative Receptor for Porcine Reproductive and Respiratory Syndrome Virus on Porcine Alveolar Macrophages

Xiaobo Duan, Hans J. Nauwynck, Herman W. Favoreel, and Maurice B. Pensaert*

Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium

Received 16 October 1997/Accepted 6 February 1998

To identify the receptor which may determine the macrophage tropism of porcine reproductive and respiratory syndrome virus (PRRSV), monoclonal antibodies (MAbs) against porcine alveolar macrophages (PAM) were produced. Two MAbs (41D3 and 41D5) which completely blocked PRRSV infection of PAM were further characterized. It was found that they reduce the attachment of PRRSV to PAM and immunoprecipitate a 210-kDa membrane protein from PAM. This protein was detected on the cell membranes of PAM but not of PRRSV-nonpermissive cells. A colocalization was found between the reactive sites of MAb 41D3 and PRRSV on PAM membranes. All PRRSV-infected cells in tissues of experimentally infected pigs reacted with MAb 41D3. Taken together, all these data suggest that the identified 210-kDa membrane protein is a putative receptor for PRRSV on porcine macrophages.

* Corresponding author. Mailing address: Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 9 264 73 66. Fax: 32 9 264 74 95. E-mail: maurice.pensaert{at}allserv.rug.ac.be.

J Virol, May 1998, p. 4520-4523, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Van Gorp, H., Van Breedam, W., Delputte, P. L., Nauwynck, H. J. (2008). Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus. J. Gen. Virol. 89: 2943-2953 [Abstract] [Full Text]  
  • Mulupuri, P., Zimmerman, J. J., Hermann, J., Johnson, C. R., Cano, J. P., Yu, W., Dee, S. A., Murtaugh, M. P. (2008). Antigen-Specific B-Cell Responses to Porcine Reproductive and Respiratory Syndrome Virus Infection. J. Virol. 82: 358-370 [Abstract] [Full Text]  
  • Delputte, P. L., Van Breedam, W., Delrue, I., Oetke, C., Crocker, P. R., Nauwynck, H. J. (2007). Porcine Arterivirus Attachment to the Macrophage-Specific Receptor Sialoadhesin Is Dependent on the Sialic Acid-Binding Activity of the N-Terminal Immunoglobulin Domain of Sialoadhesin. J. Virol. 81: 9546-9550 [Abstract] [Full Text]  
  • Calvert, J. G., Slade, D. E., Shields, S. L., Jolie, R., Mannan, R. M., Ankenbauer, R. G., Welch, S.-K. W. (2007). CD163 Expression Confers Susceptibility to Porcine Reproductive and Respiratory Syndrome Viruses. J. Virol. 81: 7371-7379 [Abstract] [Full Text]  
  • Delputte, P. L., Costers, S., Nauwynck, H. J. (2005). Analysis of porcine reproductive and respiratory syndrome virus attachment and internalization: distinctive roles for heparan sulphate and sialoadhesin. J. Gen. Virol. 86: 1441-1445 [Abstract] [Full Text]  
  • Delputte, P. L., Nauwynck, H. J. (2004). Porcine Arterivirus Infection of Alveolar Macrophages Is Mediated by Sialic Acid on the Virus. J. Virol. 78: 8094-8101 [Abstract] [Full Text]  
  • Vanderheijden, N., Delputte, P. L., Favoreel, H. W., Vandekerckhove, J., Van Damme, J., van Woensel, P. A., Nauwynck, H. J. (2003). Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages. J. Virol. 77: 8207-8215 [Abstract] [Full Text]  
  • Delputte, P. L., Vanderheijden, N., Nauwynck, H. J., Pensaert, M. B. (2002). Involvement of the Matrix Protein in Attachment of Porcine Reproductive and Respiratory Syndrome Virus to a Heparinlike Receptor on Porcine Alveolar Macrophages. J. Virol. 76: 4312-4320 [Abstract] [Full Text]  
  • Labarque, G. G., Nauwynck, H. J., Van Reeth, K., Pensaert, M. B. (2000). Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs. J. Gen. Virol. 81: 1327-1334 [Abstract] [Full Text]  
  • Fox, J. M., Bloom, M. E. (1999). Identification of a Cell Surface Protein from Crandell Feline Kidney Cells That Specifically Binds Aleutian Mink Disease Parvovirus. J. Virol. 73: 3835-3842 [Abstract] [Full Text]  
  • Nauwynck, H., Duan, X, Favoreel, H., Van Oostveldt, P, Pensaert, M. (1999). Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-mediated endocytosis. J. Gen. Virol. 80: 297-305 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»