This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Akiyoshi, D. E.
Right arrow Articles by Fishman, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akiyoshi, D. E.
Right arrow Articles by Fishman, J. A.

 Previous Article  |  Next Article 

J Virol, May 1998, p. 4503-4507, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine

Donna E. Akiyoshi,1 Maria Denaro,1 Haihong Zhu,1 Julia L. Greenstein,1 Papia Banerjee,1 and Jay A. Fishman2,*

BioTransplant, Incorporated, Charlestown,1 and Infectious Disease and Transplant Units, Massachusetts General Hospital and Harvard Medical School, Boston,2 Massachusetts

Received 20 August 1997/Accepted 15 January 1998

Endogenous retroviruses of swine are a concern in the use of pig-derived tissues for xenotransplantation into humans. The nucleotide sequence of porcine endogenous retrovirus taken from lymphocytes of miniature swine (PERV-MSL) has been characterized. PERV-MSL is a type C retrovirus of 8,132 bp with the greatest nucleic acid sequence identity to gibbon ape leukemia virus and murine leukemia virus. Constitutive production of PERV-MSL RNA has been detected in normal leukocytes and in multiple organs of swine. The copy numbers of full-length PERV sequences per genome (approximately 8 to 15) vary among swine strains. The open reading frames for gag, pol, and env in PERV-MSL have over 99% amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV). Most of the differences in the predicted amino acid sequences of PK15-ERV and PERV-MSL are in the SU (cell attach- ment) region of env. The existence of these PERV clones will enable studies of infection by endogenous retroviruses in xenotransplantation.

* Corresponding author. Mailing address: Infectious Disease Unit, Massachusetts General Hospital, 149 13th St., Charlestown, MA 02129. Phone: (617) 726-5777. Fax: (617) 726-5411. E-mail: jfishman{at}helix.mgh.harvard.edu.

J Virol, May 1998, p. 4503-4507, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Argaw, T., Figueroa, M., Salomon, D. R., Wilson, C. A. (2008). Identification of Residues outside of the Receptor Binding Domain That Influence the Infectivity and Tropism of Porcine Endogenous Retrovirus. J. Virol. 82: 7483-7491 [Abstract] [Full Text]  
  • Xiao, R., Park, K., Lee, H., Kim, J., Park, C. (2008). Identification and Classification of Endogenous Retroviruses in Cattle. J. Virol. 82: 582-587 [Abstract] [Full Text]  
  • Moalic, Y., Blanchard, Y., Felix, H., Jestin, A. (2006). Porcine Endogenous Retrovirus Integration Sites in the Human Genome: Features in Common with Those of Murine Leukemia Virus. J. Virol. 80: 10980-10988 [Abstract] [Full Text]  
  • Preuss, T., Fischer, N., Boller, K., Tonjes, R. R. (2006). Isolation and Characterization of an Infectious Replication-Competent Molecular Clone of Ecotropic Porcine Endogenous Retrovirus Class C. J. Virol. 80: 10258-10261 [Abstract] [Full Text]  
  • Martina, Y., Marcucci, K. T., Cherqui, S., Szabo, A., Drysdale, T., Srinivisan, U., Wilson, C. A., Patience, C., Salomon, D. R. (2006). Mice Transgenic for a Human Porcine Endogenous Retrovirus Receptor Are Susceptible to Productive Viral Infection. J. Virol. 80: 3135-3146 [Abstract] [Full Text]  
  • Niebert, M., Tonjes, R. R. (2005). Evolutionary Spread and Recombination of Porcine Endogenous Retroviruses in the Suiformes. J. Virol. 79: 649-654 [Abstract] [Full Text]  
  • Bartosch, B., Stefanidis, D., Myers, R., Weiss, R., Patience, C., Takeuchi, Y. (2004). Evidence and Consequence of Porcine Endogenous Retrovirus Recombination. J. Virol. 78: 13880-13890 [Abstract] [Full Text]  
  • Lavillette, D., Kabat, D. (2004). Porcine Endogenous Retroviruses Infect Cells Lacking Cognate Receptors by an Alternative Pathway: Implications for Retrovirus Evolution and Xenotransplantation. J. Virol. 78: 8868-8877 [Abstract] [Full Text]  
  • Shen, C.-H., Steiner, L. A. (2004). Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish. J. Virol. 78: 899-911 [Abstract] [Full Text]  
  • Quinn, G., Wood, J., Suling, K., Arn, S., Sachs, D. H., Schuurman, H.-J., Patience, C. (2004). Genotyping of Porcine Endogenous Retroviruses from a Family of Miniature Swine. J. Virol. 78: 314-319 [Abstract] [Full Text]  
  • Squire, T. L., Andrews, M. T. (2003). Pancreatic triacylglycerol lipase in a hibernating mammal. I. Novel genomic organization. Physiol. Genomics 16: 119-130 [Abstract] [Full Text]  
  • Tonjes, R. R., Niebert, M. (2003). Relative Age of Proviral Porcine Endogenous Retrovirus Sequences in Sus scrofa Based on the Molecular Clock Hypothesis. J. Virol. 77: 12363-12368 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2003). Characterization of Endogenous Retroviruses in Sheep. J. Virol. 77: 11268-11273 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2003). Recombination analysis of human-tropic porcine endogenous retroviruses. J. Gen. Virol. 84: 2729-2734 [Abstract] [Full Text]  
  • Wilson, C. A., Laeeq, S., Ritzhaupt, A., Colon-Moran, W., Yoshimura, F. K. (2003). Sequence Analysis of Porcine Endogenous Retrovirus Long Terminal Repeats and Identification of Transcriptional Regulatory Regions. J. Virol. 77: 142-149 [Abstract] [Full Text]  
  • Scheef, G., Fischer, N., Flory, E., Schmitt, I., Tonjes, R. R. (2002). Transcriptional Regulation of Porcine Endogenous Retroviruses Released from Porcine and Infected Human Cells by Heterotrimeric Protein Complex NF-Y and Impact of Immunosuppressive Drugs. J. Virol. 76: 12553-12563 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2002). Characterization of Porcine Endogenous Retrovirus {gamma}pro-pol Nucleotide Sequences. J. Virol. 76: 11738-11743 [Abstract] [Full Text]  
  • Bartosch, B., Weiss, R. A., Takeuchi, Y. (2002). PCR-based cloning and immunocytological titration of infectious porcine endogenous retrovirus subgroup A and B. J. Gen. Virol. 83: 2231-2240 [Abstract] [Full Text]  
  • Blusch, J. H., Seelmeir, S., von der Helm, K. (2002). Molecular and Enzymatic Characterization of the Porcine Endogenous Retrovirus Protease. J. Virol. 76: 7913-7917 [Abstract] [Full Text]  
  • Lee, J.-H., Webb, G. C., Allen, R. D. M., Moran, C. (2002). Characterizing and Mapping Porcine Endogenous Retroviruses in Westran Pigs. J. Virol. 76: 5548-5556 [Abstract] [Full Text]  
  • Niebert, M., Rogel-Gaillard, C., Chardon, P., Tonjes, R. R. (2002). Characterization of Chromosomally Assigned Replication-Competent Gamma Porcine Endogenous Retroviruses Derived from a Large White Pig and Expression in Human Cells. J. Virol. 76: 2714-2720 [Abstract] [Full Text]  
  • Herring, C., Quinn, G., Bower, R., Parsons, N., Logan, N. A., Brawley, A., Elsome, K., Whittam, A., Fernandez-Suarez, X. M., Cunningham, D., Onions, D., Langford, G., Scobie, L. (2001). Mapping Full-Length Porcine Endogenous Retroviruses in a Large White Pig. J. Virol. 75: 12252-12265 [Abstract] [Full Text]  
  • Bauer, V. W., Squire, T. L., Lowe, M. E., Andrews, M. T. (2001). Expression of a chimeric retroviral-lipase mRNA confers enhanced lipolysis in a hibernating mammal. Am. J. Physiol. Regul. Integr. Comp. Physiol. 281: R1186-R1192 [Abstract] [Full Text]  
  • Mang, R., Maas, J., Chen, X., Goudsmit, J., van der Kuyl, A. C. (2001). Identification of a novel type C porcine endogenous retrovirus: evidence that copy number of endogenous retroviruses increases during host inbreeding. J. Gen. Virol. 82: 1829-1834 [Abstract] [Full Text]  
  • Scheef, G., Fischer, N., Krach, U., Tonjes, R. R. (2001). The Number of a U3 Repeat Box Acting as an Enhancer in Long Terminal Repeats of Polytropic Replication-Competent Porcine Endogenous Retroviruses Dynamically Fluctuates during Serial Virus Passages in Human Cells. J. Virol. 75: 6933-6940 [Abstract] [Full Text]  
  • Krach, U., Fischer, N., Czauderna, F., Tonjes, R. R. (2001). Comparison of Replication-Competent Molecular Clones of Porcine Endogenous Retrovirus Class A and Class B Derived from Pig and Human Cells. J. Virol. 75: 5465-5472 [Abstract] [Full Text]  
  • Takefman, D. M., Wong, S., Maudru, T., Peden, K., Wilson, C. A. (2001). Detection and Characterization of Porcine Endogenous Retrovirus in Porcine Plasma and Porcine Factor VIII. J. Virol. 75: 4551-4557 [Abstract] [Full Text]  
  • Chapman, L. E., Bloom, E. T. (2001). Clinical Xenotransplantation. JAMA 285: 2304-2306 [Full Text]  
  • Specke, V., Tacke, S. J., Boller, K., Schwendemann, J., Denner, J. (2001). Porcine endogenous retroviruses: in vitro host range and attempts to establish small animal models. J. Gen. Virol. 82: 837-844 [Abstract] [Full Text]  
  • Ericsson, T., Oldmixon, B., Blomberg, J., Rosa, M., Patience, C., Andersson, G. (2001). Identification of Novel Porcine Endogenous Betaretrovirus Sequences in Miniature Swine. J. Virol. 75: 2765-2770 [Abstract] [Full Text]  
  • Patience, C., Switzer, W. M., Takeuchi, Y., Griffiths, D. J., Goward, M. E., Heneine, W., Stoye, J. P., Weiss, R. A. (2001). Multiple Groups of Novel Retroviral Genomes in Pigs and Related Species. J. Virol. 75: 2771-2775 [Abstract] [Full Text]  
  • Qari, S. H., Magre, S., Garcia-Lerma, J. G., Hussain, A. I., Takeuchi, Y., Patience, C., Weiss, R. A., Heneine, W. (2001). Susceptibility of the Porcine Endogenous Retrovirus to Reverse Transcriptase and Protease Inhibitors. J. Virol. 75: 1048-1053 [Abstract] [Full Text]  
  • Boneva, R. S., Folks, T. M., Chapman, L. E. (2001). Infectious Disease Issues in Xenotransplantation. Clin. Microbiol. Rev. 14: 1-14 [Abstract] [Full Text]  
  • Fishman, J. A (2000). Infection in xenotransplantation. BMJ 321: 717-718 [Full Text]  
  • Bosch, S., Arnauld, C., Jestin, A. (2000). Study of Full-Length Porcine Endogenous Retrovirus Genomes with Envelope Gene Polymorphism in a Specific-Pathogen-Free Large White Swine Herd. J. Virol. 74: 8575-8581 [Abstract] [Full Text]  
  • Czauderna, F., Fischer, N., Boller, K., Kurth, R., Tonjes, R. R. (2000). Establishment and Characterization of Molecular Clones of Porcine Endogenous Retroviruses Replicating on Human Cells. J. Virol. 74: 4028-4038 [Abstract] [Full Text]  
  • Wilson, C. A., Wong, S., VanBrocklin, M., Federspiel, M. J. (2000). Extended Analysis of the In Vitro Tropism of Porcine Endogenous Retrovirus. J. Virol. 74: 49-56 [Abstract] [Full Text]  
  • Takeuchi, Y., Patience, C., Magre, S., Weiss, R. A., Banerjee, P. T., Le Tissier, P., Stoye, J. P. (1998). Host Range and Interference Studies of Three Classes of Pig Endogenous Retrovirus. J. Virol. 72: 9986-9991 [Abstract] [Full Text]  
  • Weiss, R. A (1998). Science, medicine, and the future: Xenotransplantation. BMJ 317: 931-934 [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»