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J Virol, May 1998, p. 4503-4507, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine

Donna E. Akiyoshi,1 Maria Denaro,1 Haihong Zhu,1 Julia L. Greenstein,1 Papia Banerjee,1 and Jay A. Fishman2,*

BioTransplant, Incorporated, Charlestown,1 and Infectious Disease and Transplant Units, Massachusetts General Hospital and Harvard Medical School, Boston,2 Massachusetts

Received 20 August 1997/Accepted 15 January 1998

Endogenous retroviruses of swine are a concern in the use of pig-derived tissues for xenotransplantation into humans. The nucleotide sequence of porcine endogenous retrovirus taken from lymphocytes of miniature swine (PERV-MSL) has been characterized. PERV-MSL is a type C retrovirus of 8,132 bp with the greatest nucleic acid sequence identity to gibbon ape leukemia virus and murine leukemia virus. Constitutive production of PERV-MSL RNA has been detected in normal leukocytes and in multiple organs of swine. The copy numbers of full-length PERV sequences per genome (approximately 8 to 15) vary among swine strains. The open reading frames for gag, pol, and env in PERV-MSL have over 99% amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV). Most of the differences in the predicted amino acid sequences of PK15-ERV and PERV-MSL are in the SU (cell attach- ment) region of env. The existence of these PERV clones will enable studies of infection by endogenous retroviruses in xenotransplantation.

* Corresponding author. Mailing address: Infectious Disease Unit, Massachusetts General Hospital, 149 13th St., Charlestown, MA 02129. Phone: (617) 726-5777. Fax: (617) 726-5411. E-mail: jfishman{at}helix.mgh.harvard.edu.

J Virol, May 1998, p. 4503-4507, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.