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J Virol, May 1998, p. 4492-4497, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Viral Proteins after Infection of Cultured Hepatocytes with Rabbit Hemorrhagic Disease Virus

Matthias König,dagger Heinz-Jürgen Thiel,dagger and Gregor Meyers*

Department of Clinical Virology, Federal Research Centre for Virus Diseases of Animals, D-72001 Tübingen, Germany

Received 15 October 1997/Accepted 28 January 1998

The calicivirus rabbit hemorrhagic disease virus (RHDV), which replicates predominantly in the livers of infected rabbits, cannot be propagated in tissue culture. To enable the performance of in vitro studies, rabbit hepatocytes were isolated by liver perfusion and gradient centrifugation. After inoculation with purified RHDV, more than 50% of the cells proved to be infected. Protein analyses led to the detection of 13 RHDV-specific polypeptides within the infected cells. These proteins were assigned to defined regions of the viral genome, resulting in a refined model of RHDV genome organization.

* Corresponding author. Mailing address: Department of Clinical Virology, Federal Research Centre for Virus Diseases of Animals, P.O. Box 1149, D-72001 Tübingen, Germany. Phone: 49 7071-967207. Fax: 49 7071-967303. E-mail: gregor.meyers{at}tue.bfav.de.

dagger Present address: Institut für Virologie, FB Veterinärmedizin, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.

J Virol, May 1998, p. 4492-4497, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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