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J Virol, May 1998, p. 4434-4441, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Different Patterns of Neuronal Infection after Intracerebral Injection of Two Strains of Pseudorabies Virus

J. Patrick Card,1,2,* Pat Levitt,3 and Lynn W. Enquist4

Departments of Neuroscience,1 Neurobiology,3 and Psychiatry,2 University of Pittsburgh, Pittsburgh, Pennsylvania 15260, and Department of Molecular Biology, Princeton University, Princeton, New Jersey 085444

Received 6 October 1997/Accepted 5 February 1998

Pseudorabies virus (PRV), a swine neurotropic alphaherpesvirus, is known to invade the central nervous system (CNS) of a variety of animal species through peripherally projecting axons, replicate in the parent neurons, and then pass transsynaptically to infect other neurons of a circuit. Studies of the human pathogen herpes simplex virus type 1 have reported differences in the direction of transport of two strains of this virus after direct injection into the primate motor cortex. In the present study we examined the direction of transport of virulent and attenuated strains of PRV, utilizing injections into the rat prefrontal cortex to evaluate specific movement of virus through CNS circuitry. The data demonstrate strain-dependent patterns of infection consistent with bidirectional (anterograde and retrograde) transport of virulent virus and unidirectional (retrograde) transport of attenuated PRV from the site of injection. The distribution of infected neurons and the extent of transsynaptic passage also suggest that a release defect in the attenuated strain reduces the apparent rate of viral transport through neuronal circuitry. Finally, injection of different concentrations of virus influenced the onset of replication within a neural circuit. Taken together, these data suggest that viral envelope glycoproteins and virus concentration at the site of injection are important determinants of the rate and direction of viral transport through a multisynaptic circuit in the CNS.

* Corresponding author. Mailing address: Department of Neuroscience, 446 Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260. Phone: (412) 624-6995. Fax: (412) 624-9198. E-mail: Card{at}bns.pitt.edu.

J Virol, May 1998, p. 4434-4441, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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