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J Virol, May 1998, p. 4421-4426, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Biological Characterization of Rev Variation in Equine Infectious Anemia Virus

Michael Belshan,1 Matthew E. Harris,2 Anne E. Shoemaker,1 Thomas J. Hope,2 and Susan Carpenter1,*

Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames, Iowa 50011,1 and Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 920372

Received 14 October 1997/Accepted 23 January 1998

Sequence analysis identified significant variation in the second exon of equine infectious anemia virus (EIAV) rev. Functional analysis indicated that limited amino acid variation in Rev significantly altered the export activity of the protein but did not affect Rev-dependent alternative splicing. EIAV Rev can mediate export through two independent cis-acting Rev-responsive elements (RREs), and differences among Rev variants were more pronounced when both RREs were present. Variation in Rev may be an important mechanism for regulation of virus replication in vivo and may contribute to changes in clinical disease.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames, IA 50011. Phone: (515) 294-5158. Fax: (515) 294-8500. E-mail: scarp{at}iastate.edu.

J Virol, May 1998, p. 4421-4426, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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