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J Virol, May 1998, p. 4237-4242, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of a Novel Bovine Lymphotropic Herpesvirus

Joel Rovnak,1 Sandra L. Quackenbush,1 Richard A. Reyes,2,dagger Joel D. Baines,1 Colin R. Parrish,3 and James W. Casey1,*

Department of Microbiology and Immunology1 and The James A. Baker Institute for Animal Health,3 New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, and Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-16712

Received 26 November 1997/Accepted 22 January 1998

Degenerate PCR primers which amplify a conserved region of the DNA polymerase genes of the herpesvirus family were used to provide sequence evidence for a new bovine herpesvirus in bovine B-lymphoma cells and peripheral blood mononuclear cells (PBMC). The sequence of the resultant amplicon was found to be distinct from those of known herpesvirus isolates. Alignment of amino acid sequences demonstrated 70% identity with ovine herpesvirus 2, 69% with alcelaphine herpesvirus 1, 65% with bovine herpesvirus 4, and 42% with bovine herpesvirus 1. Phylogenetic analysis placed this putative virus within the tumorigenic Gammaherpesvirinae subfamily, and it is tentatively identified as bovine lymphotropic herpesvirus. This novel agent was expressed in vitro from infected PBMC, and cell-free supernatants were used to transfer infection to a bovine B-cell line, BL3. Analysis, with specific PCR primers, of DNA from bovine PBMC and lymphoma cells identified infection in blood of 91% of adult animals (n = 101), 63% of lymphomas (n = 32), and 38% of juveniles (n = 13). Of the adults, herpesvirus infection was present in 94% of animals that were seropositive for bovine leukemia virus (BLV) (n = 63) and in 87% of BLV-seronegative animals (n = 38). Of the seropositive group, 17 animals exhibited persistent lymphocytosis, and 100% of these were herpesvirus positive by PCR. A role for bovine lymphotropic herpesvirus as a cofactor in BLV pathogenesis is considered.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Box 5, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Phone: (607) 253-3579. Fax: (607) 253-0633. E-mail: jwc3{at}cornell.edu.

dagger Present address: Department of Medical Pathology, University of California at Davis, Davis, CA 95616.

J Virol, May 1998, p. 4237-4242, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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