This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lednicky, J. A.
Right arrow Articles by Butel, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lednicky, J. A.
Right arrow Articles by Butel, J. S.

 Previous Article  |  Next Article 

J Virol, May 1998, p. 3980-3990, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Natural Isolates of Simian Virus 40 from Immunocompromised Monkeys Display Extensive Genetic Heterogeneity: New Implications for Polyomavirus Disease

John A. Lednicky,1 Amy S. Arrington,1 A. Renee Stewart,1 Xian Min Dai,1 Connie Wong,1 Sanjeeda Jafar,1 Michael Murphey-Corb,2,dagger and Janet S. Butel1,*

Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030,1 and Tulane Regional Primate Research Center, Covington, Louisiana 704332

Received 6 November 1997/Accepted 10 February 1998

Simian virus 40 (SV40) DNAs in brain tissue and peripheral blood mononuclear cells (PBMCs) of eight simian immunodeficiency virus-infected rhesus monkeys with SV40 brain disease were analyzed. We report the detection, cloning, and identification of five new SV40 strains following a quadruple testing-verification strategy. SV40 genomes with archetypal regulatory regions (containing a duplication within the G/C-rich regulatory region segment and a single 72-bp enhancer element) were recovered from seven animal brains, two tissues of which also contained viral genomes with nonarchetypal regulatory regions (containing a duplication within the G/C-rich regulatory region segment as well as a variable duplication within the enhancer region). In contrast, PBMC DNAs from five of six animals had viral genomes with both regulatory region types. It appeared, based on T-antigen variable-region sequences, that nonarchetypal virus variants arose de novo within each animal. The eighth animal exclusively yielded a new type of SV40 strain (SV40-K661), containing a protoarchetypal regulatory region (lacking a duplication within the G/C-rich segment of the regulatory region and containing one 72-bp element in the enhancer region), from both brain tissue and PBMCs. The presence of SV40 in PBMCs suggests that hematogenous spread of viral infection may occur. An archetypal version of a virus similar to SV40 reference strain 776 (a kidney isolate) was recovered from one brain, substantiating the idea that SV40 is neurotropic as well as kidney-tropic. Indirect evidence suggests that maternal-infant transmission of SV40 may have occurred in one animal. These findings provide new insights for human polyomavirus disease.

* Corresponding author. Mailing address: Division of Molecular Virology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-3003. Fax: (713) 798-5019. E-mail: jbutel{at}bcm.tmc.edu.

dagger Present address: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.

J Virol, May 1998, p. 3980-3990, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lopez-Bueno, A., Segovia, J. C., Bueren, J. A., O'Sullivan, M. G., Wang, F., Tattersall, P., Almendral, J. M. (2008). Evolution to Pathogenicity of the Parvovirus Minute Virus of Mice in Immunodeficient Mice Involves Genetic Heterogeneity at the Capsid Domain That Determines Tropism. J. Virol. 82: 1195-1203 [Abstract] [Full Text]  
  • Sroller, V., Vilchez, R. A., Stewart, A. R., Wong, C., Butel, J. S. (2008). Influence of the Viral Regulatory Region on Tumor Induction by Simian Virus 40 in Hamsters. J. Virol. 82: 871-879 [Abstract] [Full Text]  
  • Johne, R., Muller, H. (2007). Polyomaviruses of Birds: Etiologic Agents of Inflammatory Diseases in a Tumor Virus Family. J. Virol. 81: 11554-11559 [Full Text]  
  • Johne, R., Wittig, W., Fernandez-de-Luco, D., Hofle, U., Muller, H. (2006). Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes. J. Virol. 80: 3523-3531 [Abstract] [Full Text]  
  • Cutrone, R., Lednicky, J., Dunn, G., Rizzo, P., Bocchetta, M., Chumakov, K., Minor, P., Carbone, M. (2005). Some Oral Poliovirus Vaccines Were Contaminated with Infectious SV40 after 1961. Cancer Res. 65: 10273-10279 [Abstract] [Full Text]  
  • Zhang, S., McNees, A. L., Butel, J. S. (2005). Quantification of vertical transmission of Murine polyoma virus by real-time quantitative PCR. J. Gen. Virol. 86: 2721-2729 [Abstract] [Full Text]  
  • Rubio, M.-P., Lopez-Bueno, A., Almendral, J. M. (2005). Virulent Variants Emerging in Mice Infected with the Apathogenic Prototype Strain of the Parvovirus Minute Virus of Mice Exhibit a Capsid with Low Avidity for a Primary Receptor. J. Virol. 79: 11280-11290 [Abstract] [Full Text]  
  • Johne, R., Enderlein, D., Nieper, H., Muller, H. (2005). Novel Polyomavirus Detected in the Feces of a Chimpanzee by Nested Broad-Spectrum PCR. J. Virol. 79: 3883-3887 [Abstract] [Full Text]  
  • Dang, X., Axthelm, M. K., Letvin, N. L., Koralnik, I. J. (2005). Rearrangement of Simian Virus 40 Regulatory Region Is Not Required for Induction of Progressive Multifocal Leukoencephalopathy in Immunosuppressed Rhesus Monkeys. J. Virol. 79: 1361-1366 [Abstract] [Full Text]  
  • Zaragoza, C., Li, R.-m., Fahle, G. A., Fischer, S. H., Raffeld, M., Lewis, A. M. Jr., Kopp, J. B. (2005). Squirrel Monkeys Support Replication of BK Virus More Efficiently than Simian Virus 40: an Animal Model for Human BK Virus Infection. J. Virol. 79: 1320-1326 [Abstract] [Full Text]  
  • Forsman, Z. H., Lednicky, J. A., Fox, G. E., Willson, R. C., White, Z. S., Halvorson, S. J., Wong, C., Lewis, A. M. Jr., Butel, J. S. (2004). Phylogenetic Analysis of Polyomavirus Simian Virus 40 from Monkeys and Humans Reveals Genetic Variation. J. Virol. 78: 9306-9316 [Abstract] [Full Text]  
  • Vilchez, R. A., Butel, J. S. (2004). Emergent Human Pathogen Simian Virus 40 and Its Role in Cancer. Clin. Microbiol. Rev. 17: 495-508 [Abstract] [Full Text]  
  • Zhang, S., Magnusson, G. (2003). Cellular Mobile Genetic Elements in the Regulatory Region of the Pneumotropic Mouse Polyomavirus Genome: Structure and Function in Viral Gene Expression and DNA Replication. J. Virol. 77: 3477-3486 [Abstract] [Full Text]  
  • Vivaldi, A., Pacini, F., Martini, F., Iaccheri, L., Pezzetti, F., Elisei, R., Pinchera, A., Faviana, P., Basolo, F., Tognon, M. (2003). Simian Virus 40-Like Sequences from Early and Late Regions in Human Thyroid Tumors of Different Histotypes. J. Clin. Endocrinol. Metab. 88: 892-899 [Abstract] [Full Text]  
  • Dolcetti, R., Martini, F., Quaia, M., Gloghini, A., Vignocchi, B., Cariati, R., Martinelli, M., Carbone, A., Boiocchi, M., Tognon, M. (2003). Simian Virus 40 Sequences in Human Lymphoblastoid B-Cell Lines. J. Virol. 77: 1595-1597 [Abstract] [Full Text]  
  • Li, R.-M., Branton, M. H., Tanawattanacharoen, S., Falk, R. A., Jennette, J. C., Kopp, J. B. (2002). Molecular Identification of SV40 Infection in Human Subjects and Possible Association with Kidney Disease. J. Am. Soc. Nephrol. 13: 2320-2330 [Abstract] [Full Text]  
  • Lednicky, J. A., Halvorson, S. J., Butel, J. S. (2002). PCR Detection and DNA Sequence Analysis of the Regulatory Region of Lymphotropic Papovavirus in Peripheral Blood Mononuclear Cells of an Immunocompromised Rhesus Macaque. J. Clin. Microbiol. 40: 1056-1059 [Abstract] [Full Text]  
  • Bofill-Mas, S., Pina, S., Girones, R. (2000). Documenting the Epidemiologic Patterns of Polyomaviruses in Human Populations by Studying Their Presence in Urban Sewage. Appl. Environ. Microbiol. 66: 238-245 [Abstract] [Full Text]  
  • Rizzo, P., Resta, I. D., Powers, A., Ratner, H., Carbone, M. (1999). Unique Strains of SV40 in Commercial Poliovaccines from 1955 Not Readily Identifiable with Current Testing for SV40 Infection. Cancer Res. 59: 6103-6108 [Abstract] [Full Text]  
  • Simon, M. A., Ilyinskii, P. O., Baskin, G. B., Knight, H. Y., Pauley, D. R., Lackner, A. A. (1999). Association of Simian Virus 40 with a Central Nervous System Lesion Distinct from Progressive Multifocal Leukoencephalopathy in Macaques with AIDS. Am. J. Pathol. 154: 437-446 [Abstract] [Full Text]  
  • Butel, J. S., Lednicky, J. A. (1999). Cell and Molecular Biology of Simian Virus 40: Implications for Human Infections and Disease. JNCI J Natl Cancer Inst 91: 119a-134a [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»