This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhu, M.
Right arrow Articles by Broyles, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhu, M.
Right arrow Articles by Broyles, S. S.

 Previous Article  |  Next Article 

J Virol, May 1998, p. 3893-3899, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Cellular Protein Binds Vaccinia Virus Late Promoters and Activates Transcription In Vitro

Min Zhu, Trisha Moore, and Steven S. Broyles*

Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907

Received 22 September 1997/Accepted 26 January 1998

Available evidence indicates that the transcription of the late class of vaccinia virus genes requires the participation of several virus-encoded proteins in addition to the viral RNA polymerase. In this report we describe the identification of a protein present in extracts of uninfected HeLa cells that binds avidly to viral late promoter DNA. The protein bound specifically to several different vaccinia virus late promoters but not an early nor an intermediate promoter. DNase I footprinting localized the protein's binding site to nucleotides surrounding the transcriptional start site of the I1L promoter. Optimal promoter binding required sequences in the highly conserved TAAAT motif at the transcriptional start site as well as sequences immediately upstream; however, one variation on the motif's sequence did not affect promoter binding by the protein. Partially purified late promoter binding protein (LPBP) was capable of stimulating the transcription activity of extracts depleted of LPBP on a late promoter-driven template, establishing LPBP as a transcription activator in vitro. These results suggest that a cellular protein is responsible for targeting vaccinia virus late promoters for initiation of transcription.

* Corresponding author. Mailing address: Department of Biochemistry, Purdue University, West Lafayette, IN 47907-1153. Phone: (765) 494-0745. Fax: (765) 494-7897. E-mail: broyles{at}biochem.purdue.edu.

J Virol, May 1998, p. 3893-3899, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Knutson, B. A., Oh, J., Broyles, S. S. (2009). Downregulation of vaccinia virus intermediate and late promoters by host transcription factor YY1. J. Gen. Virol. 90: 1592-1599 [Abstract] [Full Text]  
  • Mohamed, M. R., Niles, E. G. (2003). UUUUUNU Stimulation of Vaccinia Virus Early Gene Transcription Termination: OLIGONUCLEOTIDE SEQUENCE AND STRUCTURAL REQUIREMENTS FOR STIMULATION OF PREMATURE TERMINATION IN VITRO. J. Biol. Chem. 278: 39534-39541 [Abstract] [Full Text]  
  • Lackner, C. A., Condit, R. C. (2000). Vaccinia Virus Gene A18R DNA Helicase Is a Transcript Release Factor. J. Biol. Chem. 275: 1485-1494 [Abstract] [Full Text]  
  • Broyles, S. S., Liu, X., Zhu, M., Kremer, M. (1999). Transcription Factor YY1 Is a Vaccinia Virus Late Promoter Activator. J. Biol. Chem. 274: 35662-35667 [Abstract] [Full Text]  
  • Sanz, P., Moss, B. (1999). Identification of a transcription factor, encoded by two vaccinia virus early genes, that regulates the intermediate stage of viral gene expression. Proc. Natl. Acad. Sci. USA 96: 2692-2697 [Abstract] [Full Text]  
  • Gunasinghe, S. K., Hubbs, A. E., Wright, C. F. (1998). A Vaccinia Virus Late Transcription Factor with Biochemical and Molecular Identity to a Human Cellular Protein. J. Biol. Chem. 273: 27524-27530 [Abstract] [Full Text]  
  • Mohamed, M. R., Niles, E. G. (2000). Interaction between Nucleoside Triphosphate Phosphohydrolase I and the H4L Subunit of the Viral RNA Polymerase Is Required for Vaccinia Virus Early Gene Transcript Release. J. Biol. Chem. 275: 25798-25804 [Abstract] [Full Text]  
  • Mohamed, M. R., Niles, E. G. (2001). The Viral RNA Polymerase H4L Subunit Is Required for Vaccinia Virus Early Gene Transcription Termination. J. Biol. Chem. 276: 20758-20765 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»