This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mirazimi, A.
Right arrow Articles by Svensson, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mirazimi, A.
Right arrow Articles by Svensson, L.

 Previous Article  |  Next Article 

J Virol, May 1998, p. 3887-3892, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7

Ali Mirazimi and Lennart Svensson*

Department of Virology, SMI/Karolinska Institute, 105 21 Stockholm, Sweden

Received 6 October 1997/Accepted 5 February 1998

It is well established that glycosylation is essential for assembly of enveloped viruses, but no information is yet available as to the function of carbohydrates on the nonenveloped but glycosylated rotavirus. We show that tunicamycin and, more pronouncedly, a combination of tunicamycin and brefeldin A treatment caused misfolding of the luminal VP7 protein, leading to interdisulfide bond aggregation. While formation of VP7 aggregates could be prevented under reducing conditions, they reoccurred in less than 30 min after a shift to an oxidizing milieu. Furthermore, while glycosylated VP7 interacted during maturation with protein disulfide isomerase, nonglycosylated VP7 did not, suggesting that glycosylation is a prerequisite for protein disulfide isomerase interaction. While native NSP4, which does not possess S-S bonds, was not dependent on N-linked glycosylation or on protein disulfide isomerase assistance for maturation, nonglycosylated NSP4 was surprisingly found to interact with protein disulfide isomerase, further suggesting that protein disulfide isomerase can act both as an enzyme and as a chaperone. In conclusion, our data suggest that the major function of carbohydrates on VP7 is to facilitate correct disulfide bond formation and protein folding.

* Corresponding author. Mailing address: Department of Virology, SMI/Karolinska Institute, 105 21 Stockholm, Sweden. Phone: 46-8-735 12 28. Fax: 46-8-470 56 13. E-mail: Lensve{at}mbox.ki.se.

J Virol, May 1998, p. 3887-3892, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maruri-Avidal, L., Lopez, S., Arias, C. F. (2008). Endoplasmic Reticulum Chaperones Are Involved in the Morphogenesis of Rotavirus Infectious Particles. J. Virol. 82: 5368-5380 [Abstract] [Full Text]  
  • Cuadras, M. A., Bordier, B. B., Zambrano, J. L., Ludert, J. E., Greenberg, H. B. (2006). Dissecting Rotavirus Particle-Raft Interaction with Small Interfering RNAs: Insights into Rotavirus Transit through the Secretory Pathway.. J. Virol. 80: 3935-3946 [Abstract] [Full Text]  
  • Delmas, O., Durand-Schneider, A.-M., Cohen, J., Colard, O., Trugnan, G. (2004). Spike Protein VP4 Assembly with Maturing Rotavirus Requires a Postendoplasmic Reticulum Event in Polarized Caco-2 Cells. J. Virol. 78: 10987-10994 [Abstract] [Full Text]  
  • Mirazimi, A., Magnusson, K.-E., Svensson, L. (2003). A cytoplasmic region of the NSP4 enterotoxin of rotavirus is involved in retention in the endoplasmic reticulum. J. Gen. Virol. 84: 875-883 [Abstract] [Full Text]  
  • Mirazimi, A., Svensson, L. (2000). ATP Is Required for Correct Folding and Disulfide Bond Formation of Rotavirus VP7. J. Virol. 74: 8048-8052 [Abstract] [Full Text]  
  • Mirazimi, A., Mousavi-Jazi, M., Sundqvist, V.-A., Svensson, L. (1999). Free thiol groups are essential for infectivity of human cytomegalovirus. J. Gen. Virol. 80: 2861-2865 [Abstract] [Full Text]  
  • Zafrullah, M., Ozdener, M. H., Kumar, R., Panda, S. K., Jameel, S. (1999). Mutational Analysis of Glycosylation, Membrane Translocation, and Cell Surface Expression of the Hepatitis E Virus ORF2 Protein. J. Virol. 73: 4074-4082 [Abstract] [Full Text]  
  • Xu, A., Bellamy, A. R., Taylor, J. A. (1998). BiP (GRP78) and Endoplasmin (GRP94) Are Induced following Rotavirus Infection and Bind Transiently to an Endoplasmic Reticulum-Localized Virion Component. J. Virol. 72: 9865-9872 [Abstract] [Full Text]  
  • Mirazimi, A., Nilsson, M., Svensson, L. (1998). The Molecular Chaperone Calnexin Interacts with the NSP4 Enterotoxin of Rotavirus In Vivo and In Vitro. J. Virol. 72: 8705-8709 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»