This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Apetrei, C.
Right arrow Articles by Brun-Vézinet, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Apetrei, C.
Right arrow Articles by Brun-Vézinet, F.

 Previous Article  |  Next Article 

J Virol, May 1998, p. 3534-3538, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Immunodeficiency Virus Type 1 Subtype F Reverse Transcriptase Sequence and Drug Susceptibility

Cristian Apetrei,1,2,* Diane Descamps,1 Gilles Collin,1 Ibtisam Loussert-Ajaka,1 Florence Damond,1 Mihai Duca,2 François Simon,1 and Françoise Brun-Vézinet1

Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75018 Paris, France,1 and Virus Laboratory, Microbiology Department, School of Medicine, Gr. T. Popa University, 6600 Iasi, Romania2

Received 21 August 1997/Accepted 15 January 1998

We sequenced and phylogenetically analyzed the reverse transcriptase (RT) regions of the pol genes of 14 human immunodeficiency virus type 1 (HIV-1) isolates from Romanian patients, which were classified as subtype F on the basis of env gene structure. The RT sequences showed that the strains clustered phylogenetically and were equidistant from other HIV-1 subtypes as shown by the neighbor-joining and maximum-likelihood methods, allowing us to define HIV-1 subtype F according to the pol classification. The subtype F RT sequences differed from reported group M RT sequences by 10.94% (for nucleotides) and 7.6% (for amino acids). Phenotypic analysis of subtype F susceptibility to three classes of antiretroviral compounds showed an increase in the 50% inhibitory concentration of the tetrahydroimidazo[4,5,1-jk][1,4]-benzodiazepin-2-(1H)-one and -thione (TIBO) derivate R82913 for one strain which was naturally resistant to this compound. This first report of subtype F pol sequences confirms the perfect correlation between the phylogenetic positions determined by env and pol analyses and suggests that virus variability might influence the efficacy of antiretroviral treatments. This finding warrants a global evaluation of the phenotypic and genotypic susceptibility of HIV-1 subtypes to antiretroviral drugs.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôp. Bichat-Claude Bernard, 46 Rue Henri Huchard, 75018 Paris, France. Phone: 33 1 40 25 88 94. Fax: 33 1 46 27 02 08. E-mail: francois.simon{at}bch.ap-hop-paris.fr.

J Virol, May 1998, p. 3534-3538, Vol. 72, No. 5
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Waleria-Aleixo, A., Martins, A. N., Arruda, M. B., Brindeiro, R. M., Da-Silva, R. M., Nobre, F. F., Greco, D. B., Tanuri, A. (2008). Drug Resistance Mutation Profile and Accumulation Kinetics in Human Immunodeficiency Virus-Positive Individuals Infected with Subtypes B and F Failing Highly Active Antiretroviral Therapy Are Influenced by Different Viral Codon Usage Patterns. Antimicrob. Agents Chemother. 52: 4497-4502 [Abstract] [Full Text]  
  • Ndembi, N., Abraha, A., Pilch, H., Ichimura, H., Mbanya, D., Kaptue, L., Salata, R., Arts, E. J. (2008). Molecular Characterization of Human Immunodeficiency Virus Type 1 (HIV-1) and HIV-2 in Yaounde, Cameroon: Evidence of Major Drug Resistance Mutations in Newly Diagnosed Patients Infected with Subtypes Other than Subtype B. J. Clin. Microbiol. 46: 177-184 [Abstract] [Full Text]  
  • Apetrei, C., Gautam, R., Sumpter, B., Carter, A. C., Gaufin, T., Staprans, S. I., Else, J., Barnes, M., Cao, R. Jr., Garg, S., Milush, J. M., Sodora, D. L., Pandrea, I., Silvestri, G. (2007). Virus Subtype-Specific Features of Natural Simian Immunodeficiency Virus SIVsmm Infection in Sooty Mangabeys. J. Virol. 81: 7913-7923 [Abstract] [Full Text]  
  • Hirigoyen, D. L., Cartwright, C. P. (2005). Use of Sequence Data Generated in the Bayer TruGene Genotyping Assay To Recognize and Characterize Non-Subtype-B Human Immunodeficiency Virus Type 1 Strains. J. Clin. Microbiol. 43: 5263-5271 [Abstract] [Full Text]  
  • Saunders, N. A., Alexander, S., Tatt, I. (2005). env Gene Typing of Human Immunodeficiency Virus Type 1 Strains on Electronic Microarrays. J. Clin. Microbiol. 43: 1910-1916 [Abstract] [Full Text]  
  • Eshleman, S. H., Hackett, J. Jr., Swanson, P., Cunningham, S. P., Drews, B., Brennan, C., Devare, S. G., Zekeng, L., Kaptue, L., Marlowe, N. (2004). Performance of the Celera Diagnostics ViroSeq HIV-1 Genotyping System for Sequence-Based Analysis of Diverse Human Immunodeficiency Virus Type 1 Strains. J. Clin. Microbiol. 42: 2711-2717 [Abstract] [Full Text]  
  • Nicastri, E., Sarmati, L., d'Ettorre, G., Parisi, S. G., Palmisano, L., Galluzzo, C., Montano, M., Uccella, I., Amici, R., Gatti, F., Vullo, V., Concia, E., Vella, S., Andreoni, M. (2003). High Prevalence of M184 Mutation among Patients with Viroimmunologic Discordant Responses to Highly Active Antiretroviral Therapy and Outcomes after Change of Therapy Guided by Genotypic Analysis. J. Clin. Microbiol. 41: 3007-3012 [Abstract] [Full Text]  
  • Spira, S., Wainberg, M. A., Loemba, H., Turner, D., Brenner, B. G. (2003). Impact of clade diversity on HIV-1 virulence, antiretroviral drug sensitivity and drug resistance. J Antimicrob Chemother 51: 229-240 [Abstract] [Full Text]  
  • Loemba, H., Brenner, B., Parniak, M. A., Ma'ayan, S., Spira, B., Moisi, D., Oliveira, M., Detorio, M., Wainberg, M. A. (2002). Genetic Divergence of Human Immunodeficiency Virus Type 1 Ethiopian Clade C Reverse Transcriptase (RT) and Rapid Development of Resistance against Nonnucleoside Inhibitors of RT. Antimicrob. Agents Chemother. 46: 2087-2094 [Abstract] [Full Text]  
  • Fonjungo, P. N., Mpoudi, E. N., Torimiro, J. N., Alemnji, G. A., Eno, L. T., Lyonga, E. J., Nkengasong, J. N., Lal, R. B., Rayfield, M., Kalish, M. L., Folks, T. M., Pieniazek, D. (2002). Human Immunodeficiency Virus Type 1 Group M Protease in Cameroon: Genetic Diversity and Protease Inhibitor Mutational Features. J. Clin. Microbiol. 40: 837-845 [Abstract] [Full Text]  
  • Plantier, J.-C., Vergne, L., Damond, F., MBoup, S., MPoudi-NGole, E., Buzelay, L., Farfara, I., Brand, D., Peeters, M., Brun-Vezinet, F., Delaporte, E., Barin, F. (2002). Development and Evaluation of a DNA Enzyme Immunoassay Method for env Genotyping of Subtypes A through G of Human Immunodeficiency Virus Type 1 Group M, with Discrimination of the Circulating Recombinant Forms CRF01_AE and CRF02_AG. J. Clin. Microbiol. 40: 1010-1022 [Abstract] [Full Text]  
  • Mracna, M., Becker-Pergola, G., Dileanis, J., Guay, L. A., Cunningham, S., Jackson, J. B., Eshleman, S. H. (2001). Performance of Applied Biosystems ViroSeq HIV-1 Genotyping System for Sequence-Based Analysis of Non-Subtype B Human Immunodeficiency Virus Type 1 from Uganda. J. Clin. Microbiol. 39: 4323-4327 [Abstract] [Full Text]  
  • Vidal, N., Peeters, M., Mulanga-Kabeya, C., Nzilambi, N., Robertson, D., Ilunga, W., Sema, H., Tshimanga, K., Bongo, B., Delaporte, E. (2000). Unprecedented Degree of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Genetic Diversity in the Democratic Republic of Congo Suggests that the HIV-1 Pandemic Originated in Central Africa. J. Virol. 74: 10498-10507 [Abstract] [Full Text]  
  • Vergne, L., Peeters, M., Mpoudi-Ngole, E., Bourgeois, A., Liegeois, F., Toure-Kane, C., Mboup, S., Mulanga-Kabeya, C., Saman, E., Jourdan, J., Reynes, J., Delaporte, E. (2000). Genetic Diversity of Protease and Reverse Transcriptase Sequences in Non-Subtype-B Human Immunodeficiency Virus Type 1 Strains: Evidence of Many Minor Drug Resistance Mutations in Treatment-Naive Patients. J. Clin. Microbiol. 38: 3919-3925 [Abstract] [Full Text]  
  • Quinones-Mateu, M. E., Albright, J. L., Mas, A., Soriano, V., Arts, E. J. (1998). Analysis of pol Gene Heterogeneity, Viral Quasispecies, and Drug Resistance in Individuals Infected with Group O Strains of Human Immunodeficiency Virus Type 1. J. Virol. 72: 9002-9015 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»