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J Virol, April 1998, p. 3495-3500, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The TAATGARAT Motif in the Herpes Simplex Virus Immediate-Early Gene Promoters Can Confer both Positive and Negative Responses to Cellular Octamer-Binding Proteins When It Is Located within the Viral Genome

Suzanne Thomas,1 Robert S. Coffin,1 Paul Watts,2 Gerald Gough,2 and David S. Latchman1,*

Department of Molecular Pathology, Windeyer Institute of Medical Sciences, University College London Medical School, London W1P 6DB,1 and Glaxo/Wellcome Medicines Research Centre, Stevenage, Hertfordshire,2 United Kingdom

Received 2 September 1997/Accepted 4 December 1997

The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with different IE promoters driving a reporter beta -galactosidase gene within the viral genome to investigate this. We showed that deletion of the upstream IE promoter region containing the TAATGARAT motifs abolishes the inhibitory effect of the cellular octamer-binding proteins Oct-2.4 and Oct-2.5 on the viral IE promoter. This inhibitory effect can be restored by addition of a single TAATGARAT motif to the minimal promoter within the viral genome. Hence, the TAATGARAT motif can indeed mediate both positive and negative effects of cellular transcription factors when it is located within the viral genome.

* Corresponding author. Mailing address: Department of Molecular Pathology, Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom. Phone: 171-380-9343. Fax: 171-387-3310. E-mail: d.latchman{at}ucl.ac.uk.




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