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J Virol, April 1998, p. 3455-3458, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Adenovirus Internalization and Infection
Require Dynamin
Kena
Wang,
Shuang
Huang,
Archana
Kapoor-Munshi, and
Glen
Nemerow*
Department of Immunology, The Scripps
Research Institute, La Jolla, California
Received 22 September 1997/Accepted 23 December 1997
The cell receptors that facilitate adenovirus internalization into
cells have been identified; however, the infectious pathway of virus
entry has not been established. Adenovirus entry and infection were
examined in HeLa cells lacking or overexpressing mutant dynamin, a
protein that specifically regulates clathrin-mediated endocytosis.
Expression of mutant dynamin significantly reduced adenovirus
internalization and gene delivery, indicating a functional requirement
for this molecule. These findings are consistent with virus entry via
the clathrin-coated pit pathway.
*
Corresponding author. Mailing address: Department of
Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-8072. Fax: (619) 784-8472. E-mail:
gnemerow{at}scripps.edu.

Publication 11132-IMM from The Scripps Research Institute.
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