This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rein, A.
Right arrow Articles by Compans, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rein, A.
Right arrow Articles by Compans, R. W.

 Previous Article  |  Next Article 

J Virol, April 1998, p. 3432-3435, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evidence for Cooperation between Murine Leukemia Virus Env Molecules in Mixed Oligomers

Alan Rein,1,* Chinglai Yang,2 Jacqueline A. Haynes,1,dagger Jane Mirro,1 and Richard W. Compans2

Retroviral Genetics Section, ABL Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702,1 and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 303222

Received 19 August 1997/Accepted 12 December 1997

A retroviral Env molecule consists of a surface glycoprotein (SU) complexed with a transmembrane protein (TM). In turn, these complexes are grouped into oligomers on the surfaces of the cell and of the virion. In the case of murine leukemia viruses (MuLVs), the SU moieties are polymorphic, with SU proteins of different viral isolates directed towards different cell surface receptors. During maturation of the released virus particle, the 16 C-terminal residues of TM (the R peptide or p2E) are removed from the protein by the viral protease; this cleavage is believed to activate the membrane-fusing potential of MuLV Env. We have tested the possibility that different MuLV Env proteins in the same cell can interact with each other, both physically and functionally, in mixed oligomers. We found that coexpressed Env molecules can be precipitated out of cell lysates by antiserum which reacts with only one of them. Furthermore, they can evidently cooperate with each other: if one Env species lacks the R peptide, then it can apparently induce fusion if the SU protein of the other Env species encounters its cognate receptor on the surface of another cell. This functional interaction between different Env molecules has a number of implications with respect to the mechanism of induction of membrane fusion, for the genetic analysis of Env function, and for the design of targeted retroviral vectors for gene therapy.

* Corresponding author. Mailing address: Retroviral Genetics Section, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. Box B, Bldg. 535, Rm. 211, Frederick, MD 21702. Phone: (301) 846-1361. Fax: (301) 846-7146. E-mail: rein{at}ncifcrf.gov.

dagger Present address: Vaccine Bioprocessing Research and Development, Merck and Company, West Point, PA 19486.




This article has been cited by other articles:

  • Murphy, S. L., Gaulton, G. N. (2007). TR1.3 Viral Pathogenesis and Syncytium Formation Are Linked to Env-Gag Cooperation. J. Virol. 81: 10777-10785 [Abstract] [Full Text]  
  • Ou, W., Silver, J. (2006). Stoichiometry of Murine Leukemia Virus Envelope Protein-Mediated Fusion and Its Neutralization. J. Virol. 80: 11982-11990 [Abstract] [Full Text]  
  • Carmo, M., Faria, T. Q., Falk, H., Coroadinha, A. S., Teixeira, M., Merten, O.-W., Geny-Fiamma, C., Alves, P. M., Danos, O., Panet, A., Carrondo, M. J. T., Cruz, P. E. (2006). Relationship between retroviral vector membrane and vector stability.. J. Gen. Virol. 87: 1349-1356 [Abstract] [Full Text]  
  • Song, C., Micoli, K., Bauerova, H., Pichova, I., Hunter, E. (2005). Amino Acid Residues in the Cytoplasmic Domain of the Mason-Pfizer Monkey Virus Glycoprotein Critical for Its Incorporation into Virions. J. Virol. 79: 11559-11568 [Abstract] [Full Text]  
  • Aguilar, H. C., Anderson, W. F., Cannon, P. M. (2002). Cytoplasmic Tail of Moloney Murine Leukemia Virus Envelope Protein Influences the Conformation of the Extracellular Domain: Implications for Mechanism of Action of the R Peptide. J. Virol. 77: 1281-1291 [Abstract] [Full Text]  
  • Christodoulopoulos, I., Cannon, P. M. (2001). Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus Vectors. J. Virol. 75: 4129-4138 [Abstract] [Full Text]  
  • Salzwedel, K., Berger, E. A. (2000). Cooperative subunit interactions within the oligomeric envelope glycoprotein of HIV-1: Functional complementation of specific defects in gp120 and gp41. Proc. Natl. Acad. Sci. USA 10.1073/pnas.230438497v1 [Abstract] [Full Text]  
  • Bachrach, E., Marin, M., Pelegrin, M., Karavanas, G., Piechaczyk, M. (2000). Efficient Cell Infection by Moloney Murine Leukemia Virus-Derived Particles Requires Minimal Amounts of Envelope Glycoprotein. J. Virol. 74: 8480-8486 [Abstract] [Full Text]  
  • Yang, C., Yang, Q., Compans, R. W. (2000). Coreceptor-Dependent Inhibition of the Cell Fusion Activity of Simian Immunodeficiency Virus Env Proteins. J. Virol. 74: 6217-6222 [Abstract] [Full Text]  
  • Lerner, D. L., Elder, J. H. (2000). Expanded Host Cell Tropism and Cytopathic Properties of Feline Immunodeficiency Virus Strain PPR Subsequent to Passage through Interleukin-2-Independent T Cells. J. Virol. 74: 1854-1863 [Abstract] [Full Text]  
  • Lavillette, D., Ruggieri, A., Russell, S. J., Cosset, F.-L. (2000). Activation of a Cell Entry Pathway Common to Type C Mammalian Retroviruses by Soluble Envelope Fragments. J. Virol. 74: 295-304 [Abstract] [Full Text]  
  • Schonning, K., Lund, O., Sogaard Lund, O., Stig Hansen, J.-E. (1999). Stoichiometry of Monoclonal Antibody Neutralization of T-Cell Line-Adapted Human Immunodeficiency Virus Type 1. J. Virol. 73: 8364-8370 [Abstract] [Full Text]  
  • Zavorotinskaya, T., Albritton, L. M. (1999). Failure To Cleave Murine Leukemia Virus Envelope Protein Does Not Preclude Its Incorporation in Virions and Productive Virus-Receptor Interaction. J. Virol. 73: 5621-5629 [Abstract] [Full Text]  
  • Zavorotinskaya, T., Albritton, L. M. (1999). Suppression of a Fusion Defect by Second Site Mutations in the Ecotropic Murine Leukemia Virus Surface Protein. J. Virol. 73: 5034-5042 [Abstract] [Full Text]  
  • Zhao, Y., Zhu, L., Lee, S., Li, L., Chang, E., Soong, N.-W., Douer, D., Anderson, W. F. (1999). Identification of the block in targeted retroviral-mediated gene transfer. Proc. Natl. Acad. Sci. USA 96: 4005-4010 [Abstract] [Full Text]  
  • Han, J.-Y., Zhao, Y., Anderson, W. F., Cannon, P. M. (1998). Role of Variable Regions A and B in Receptor Binding Domain of Amphotropic Murine Leukemia Virus Envelope Protein. J. Virol. 72: 9101-9108 [Abstract] [Full Text]  
  • Salzwedel, K., Berger, E. A. (2000). Cooperative subunit interactions within the oligomeric envelope glycoprotein of HIV-1: Functional complementation of specific defects in gp120 and gp41. Proc. Natl. Acad. Sci. USA 97: 12794-12799 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»