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J Virol, April 1998, p. 3423-3426, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Entire Nucleotide Sequence of Friend-Related and Paralysis-Inducing PVC-441 Murine Leukemia Virus (MuLV) and Its Comparison with Those of PVC-211 MuLV and Friend MuLV

Atsushi Tanaka,1 Kiyomasa Oka,1 Keiji Tanaka,1 Atsushi Jinno,1 Sandra K. Ruscetti,2 and Kazushige Kai1,*

Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi City 753, Japan,1 and Basic Research Laboratory, National Cancer Institute, Frederick, Maryland 21702-12012

Received 7 October 1997/Accepted 22 December 1997

PVC-441 murine leukemia virus (MuLV) is a member of the PVC group of Friend MuLV (F-MuLV)-derived neuropathogenic retroviruses. In order to determine the molecular basis for the difference in neuropathogenicity between PVC-441 and the previously characterized PVC-211 MuLVs, the entire nucleotide sequence of PVC-441 MuLV was determined and compared with those of PVC-211 and F-MuLV. The results suggest that PVC-441 and PVC-211 MuLVs were formed as a result of random mutations of F-MuLV and developed differently. The distinct pathogenicities of PVC-441 and PVC-211 MuLVs were maintained in the viruses regenerated from their molecular clones, and the sequences responsible for the pathological differences observed can be localized to the env gene. The amino acid sequence of PVC-441 deduced from its nucleotide sequence revealed a number of differences from PVC-211, the most striking of which was a difference at position 129 of the SU proteins in the two viruses. Host range studies with a brain capillary endothelial cell line (RTEC-6) and Chinese hamster ovary cells (CHO-K1) revealed that PVC-441, like PVC-211, could infect these cells but its efficiency of infection was lower than that of PVC-211. These results may account for the difference in neuropathogenicity between PVC-441 and PVC-211.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi City 753, Japan. Phone and Fax: 81-839-33-5888. E-mail: kkai{at}agr.yamaguchi-u.ac.jp.




This article has been cited by other articles:

  • Jinno-Oue, A., Oue, M., Ruscetti, S. K. (2001). A Unique Heparin-Binding Domain in the Envelope Protein of the Neuropathogenic PVC-211 Murine Leukemia Virus May Contribute to Its Brain Capillary Endothelial Cell Tropism. J. Virol. 75: 12439-12445 [Abstract] [Full Text]  


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