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J Virol, April 1998, p. 3377-3382, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Studies of the Nucleopolyhedrovirus Infection Process in Insects by Using the Green Fluorescence Protein as a Reporter

John W. Barrett, Andy J. Brownwright, Mark J. Primavera, and Subba Reddy Palli*

Great Lakes Forestry Centre, Canadian Forest Service, Sault Ste. Marie, Ontario P6A 5M7, Canada

Received 26 August 1997/Accepted 23 December 1997

A recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) expressing the green fluorescence protein (GFP) under the control of the AcMNPV polyhedrin promoter was constructed to study the spatial and temporal regulation of baculovirus infection in a permissive host. Larvae that ingested AcMNPV-GFP showed localized expression of GFP in the midgut epithelial cells, as well as hemocytes, at 24 h postinfection. The presence of fluorescence in these tissues indicated not only that the virus was replicating but also that the very late viral proteins were being synthesized. Secondary infection occurred within the tracheal cells throughout the body cavity, confirming earlier reports, and these foci of infection allowed entry of the virus into other tissues, such as the epidermis and the fat body.

* Corresponding author. Mailing address: Great Lakes Forestry Centre, 1219 Queen St. East, P.O. Box 490, Canadian Forest Service, Sault Ste. Marie, Ontario P6A 5M7, Canada. Phone: (705) 759-5740, ext. 2468. Fax: (705) 759-5700. E-mail: rpalli{at}am.glfc.forestry.ca.




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