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J Virol, April 1998, p. 3370-3376, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A Cytopathogenic, Apoptosis-Inducing Variant of Hepatitis A
Virus
Kerstin
Brack,
Werner
Frings,
Andreas
Dotzauer,* and
Angelika
Vallbracht
Department of Virology, University of Bremen,
D-28211 Bremen, Germany
Received 28 July 1997/Accepted 22 December 1997
A cytopathogenic variant of hepatitis A virus
(HAVcyt/HB1.1) was isolated from persistently infected
BS-C-1 cells by serial passages in FRhK-4 cells. This virus shows a
rapid replication pattern and high final titers are obtained, which are
main characteristics of cytopathogenic HAVs. Sequencing of the
nontranslated regions and the coding regions for 2ABC and 3AB revealed
that mutations are distributed all over these regions and that certain
mutated sites correspond to those in other cytopathogenic HAV variants. Investigating the mechanisms causing the cytopathic effect in FRhK-4
cells infected with this variant, we found that an apoptotic reaction
takes place.
*
Corresponding author. Mailing address: Department of
Virology, University of Bremen, Argonnenstr. 3, D-28211 Bremen,
Germany. Phone: 421-218-4354. Fax: 421-218-4266. E-mail:
dotzauer{at}uni-bremen.de.

This work is dedicated to H.-J. Gerth on the occasion of his
70th birthday.
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