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J Virol, April 1998, p. 3066-3071, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Novel Membrane Protein Is a Mouse Mammary Tumor Virus Receptor

Tatyana V. Golovkina,1,dagger John Dzuris,1 Bernadette van den Hoogen,1 Aron B. Jaffe,1 Paul C. Wright,2 Shelagh M. Cofer,2 and Susan R. Ross1,*

Department of Microbiology/Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6142,1 and Department of Biochemistry, University of Illinois School of Medicine, Chicago, Illinois 606122

Received 1 August 1997/Accepted 23 December 1997

Mouse mammary tumor virus (MMTV) infects a number of different cell types, including mammary gland and lymphoid cells, in vivo. To identify the cellular receptor for this virus, a mouse cDNA expression library was transfected into Cos-7 monkey kidney cells, and those transfected cells able to bind virus were selected by using antibody against the virus's cell surface envelope protein, gp52. One clone isolated from a library prepared from newborn thymus RNA, called MTVR, was able to confer virus binding to both monkey and human cells; this binding was blocked by anti-MTVR antibody. Moreover, transfection of MTVR into CV1 cells rendered them susceptible to infection by a murine leukemia virus-based retrovirus vector pseudotyped with the MMTV envelope protein. An epitope-tagged MTVR cofractionated with cellular membranes. Coimmunoprecipitation of the MMTV envelope protein and a MTVR-rabbit Fc fusion protein showed that these two proteins bound to each other. The MTVR sequence clone is unique, shows no homology to known membrane proteins, and is transcribed in many tissues.

* Corresponding author. Mailing address: Department of Microbiology/Cancer Center, University of Pennsylvania, 526 CRB, 415 Curie Blvd., Philadelphia, PA 19104-6142. Phone: (215) 898-9764. Fax: (215) 573-2028. E-mail: rosss{at}mail.med.upenn.edu.

dagger Present address: The Jackson Laboratory, Bar Harbor, Maine.




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