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J Virol, April 1998, p. 2577-2588, Vol. 72, No. 4
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mtv-1 Superantigen Trafficks Independently of Major Histocompatibility Complex Class II Directly to the B-Cell Surface by the Exocytic Pathway

Michael E. Grigg,^1,2, Christopher W. McMahon,² Stanislaw Morkowski,² Alexander Y. Rudensky,^1,2 and Ann M. Pullen^1,2,*

Howard Hughes Medical Institute¹ and Department of Immunology,² University of Washington School of Medicine, Seattle, Washington 98195

Received 13 October 1997/Accepted 22 December 1997

Presentation of the Mtv-1 superantigen (vSag1) to specific V-bearing T cells requires association with major histocompatibility complex class II molecules. The intracellular route by which vSag1 trafficks to the cell surface and the site of vSag1-class II complex assembly in antigen-presenting B lymphocytes have not been determined. Here, we show that vSag1 trafficks independently of class II to the plasma membrane by the exocytic secretory pathway. At the surface of B cells, vSag1 associates primarily with mature peptide-bound class II dimers, which are stable in sodium dodecyl sulfate. vSag1 is unstable on the cell surface in the absence of class II, and reagents that alter the surface expression of vSag1 and the conformation of class II molecules affect vSag1 stimulation of superantigen reactive T cells.

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^* Corresponding author. Present address: Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom. Phone: 44-117-9287881. Fax: 44-117-9287896. E-mail: a.m.pullen{at}bristol.ac.uk.

Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5124.

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