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J Virol, March 1998, p. 2496-2499, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Autocrine Regulation and Experimental Modulation of Interleukin-6 Expression by Human Pulmonary Epithelial Cells Infected with Respiratory Syncytial Virus

Zili Jiang, Masaru Kunimoto, and Janak A. Patel*

Division of Pediatric Infectious Diseases, Department of Pediatrics, Children's Hospital at University of Texas Medical Branch, Galveston, Texas 77555

Received 26 June 1997/Accepted 24 November 1997

The mechanisms of regulation of interleukin-6 (IL-6) production in respiratory syncytial virus (RSV)-infected respiratory epithelial cells were evaluated in A549 cell cultures. Incubation with purified RSV resulted in significant production of IL-1alpha , IL-1beta , IL-6, and tumor necrosis factor alpha (TNF-alpha ). Addition of saturating concentrations of neutralizing antibodies against IL-1alpha , IL-1beta , or TNF-alpha into purified RSV-infected cell cultures resulted in a significant inhibition of IL-6 production, although anti-IL-1alpha antibody had the most predominant effect (80% inhibition). Anti-IL-1alpha antibody also almost completely blocked the expression of mRNA for IL-6. Addition of therapeutic concentrations of dexamethasone (1 µM) or ribavirin (90 µg/ml), an antiviral agent, also significantly inhibited the synthesis of IL-6. Hence, in clinical settings, pharmacological agents such as the specific antagonists of IL-6-inducing cytokines, as well as dexamethasone and ribavirin, could be used to modulate IL-6 production.

* Corresponding author. Mailing address: Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0371. Phone: (409) 772-2798. Fax: (409) 747-1753. E-mail: janak.patel{at}utmb.edu.




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