This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rouiller, I.
Right arrow Articles by Wileman, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rouiller, I.
Right arrow Articles by Wileman, T.

 Previous Article  |  Next Article 

J Virol, March 1998, p. 2373-2387, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

African Swine Fever Virus Is Wrapped by the Endoplasmic Reticulum

Isabelle Rouiller,1 Sharon M. Brookes,1 Alex D. Hyatt,2 Miriam Windsor,1 and Thomas Wileman1,*

Division of Immunology, Pirbright Laboratories, Institute for Animal Health, Surrey, England,1 and Australian Animal Health Laboratory, Geelong, Victoria, Australia2

Received 18 August 1997/Accepted 5 December 1997

African swine fever (ASF) virus is a large DNA virus that shares the striking icosahedral symmetry of iridoviruses and the genomic organization of poxviruses. Both groups of viruses have a complex envelope structure. In this study, the mechanism of formation of the inner envelope of ASF virus was investigated. Examination of thin cryosections by electron microscopy showed two internal membranes in mature intracellular virions and all structural intermediates. These membranes were in continuity with intracellular membrane compartments, suggesting that the virus gained two membranes from intracellular membrane cisternae. Immunogold electron microscopy showed the viral structural protein p17 and resident membrane proteins of the endoplasmic reticulum (ER) within virus assembly sites, virus assembly intermediates, and mature virions. Resident ER proteins were also detected by Western blotting of isolated virions. The data suggested the ASF virus was wrapped by the ER. Analysis of the published sequence of ASF virus (R. J. Yanez et al., Virology 208:249-278, 1995) revealed a reading frame, XP124L, that encoded a protein predicted to translocate into the lumen of the ER. Pulse-chase immunoprecipitation and glycosylation analysis of pXP124L, the product of the XP124L gene, showed that pXP124L was retained in the ER lumen after synthesis. When analyzed by immunogold electron microscopy, pXP124L localized to virus assembly intermediates and fully assembled virions. Western blot analysis detected pXP124L in virions isolated from Percoll gradients. The packaging of pXP124L from the lumen of the ER into the virion is consistent with ASF virus being wrapped by ER cisternae: a mechanism which explains the presence of two membranes in the viral envelope.

* Corresponding author. Mailing address: Division of Immunology, Pirbright Laboratories, Institute for Animal Health, Ash Rd., Surrey GU24 ONF, England. Phone: 1483232441. Fax: 1483232448. E-mail: thomas.wileman{at}BBSRC.ac.uk.




This article has been cited by other articles:

  • Voehler, M. W., Eoff, R. L., McDonald, W. H., Guengerich, F. P., Stone, M. P. (2009). Modulation of the Structure, Catalytic Activity, and Fidelity of African Swine Fever Virus DNA Polymerase X by a Reversible Disulfide Switch. J. Biol. Chem. 284: 18434-18444 [Abstract] [Full Text]  
  • Hawes, P. C., Netherton, C. L., Wileman, T. E., Monaghan, P. (2008). The Envelope of Intracellular African Swine Fever Virus Is Composed of a Single Lipid Bilayer. J. Virol. 82: 7905-7912 [Abstract] [Full Text]  
  • Cobbold, C., Windsor, M., Parsley, J., Baldwin, B., Wileman, T. (2007). Reduced redox potential of the cytosol is important for African swine fever virus capsid assembly and maturation. J. Gen. Virol. 88: 77-85 [Abstract] [Full Text]  
  • Epifano, C., Krijnse-Locker, J., Salas, M. L., Rodriguez, J. M., Salas, J. (2006). The African Swine Fever Virus Nonstructural Protein pB602L Is Required for Formation of the Icosahedral Capsid of the Virus Particle. J. Virol. 80: 12260-12270 [Abstract] [Full Text]  
  • Epifano, C., Krijnse-Locker, J., Salas, M. L., Salas, J., Rodriguez, J. M. (2006). Generation of Filamentous Instead of Icosahedral Particles by Repression of African Swine Fever Virus Structural Protein pB438L. J. Virol. 80: 11456-11466 [Abstract] [Full Text]  
  • Chang, A. C. Y., Zsak, L., Feng, Y., Mosseri, R., Lu, Q., Kowalski, P., Zsak, A., Burrage, T. G., Neilan, J. G., Kutish, G. F., Lu, Z., Laegreid, W., Rock, D. L., Cohen, S. N. (2006). Phenotype-based identification of host genes required for replication of african Swine Fever virus.. J. Virol. 80: 8705-8717 [Abstract] [Full Text]  
  • Thammavongsa, V., Raghuraman, G., Filzen, T. M., Collins, K. L., Raghavan, M. (2006). HLA-B44 Polymorphisms at Position 116 of the Heavy Chain Influence TAP Complex Binding via an Effect on Peptide Occupancy.. J. Immunol. 177: 3150-3161 [Abstract] [Full Text]  
  • Cancellotti, E., Wiseman, F., Tuzi, N. L., Baybutt, H., Monaghan, P., Aitchison, L., Simpson, J., Manson, J. C. (2005). Altered Glycosylated PrP Proteins Can Have Different Neuronal Trafficking in Brain but Do Not Acquire Scrapie-like Properties. J. Biol. Chem. 280: 42909-42918 [Abstract] [Full Text]  
  • Stefanovic, S., Windsor, M., Nagata, K.-i., Inagaki, M., Wileman, T. (2005). Vimentin Rearrangement during African Swine Fever Virus Infection Involves Retrograde Transport along Microtubules and Phosphorylation of Vimentin by Calcium Calmodulin Kinase II. J. Virol. 79: 11766-11775 [Abstract] [Full Text]  
  • Moffat, K., Howell, G., Knox, C., Belsham, G. J., Monaghan, P., Ryan, M. D., Wileman, T. (2005). Effects of Foot-and-Mouth Disease Virus Nonstructural Proteins on the Structure and Function of the Early Secretory Pathway: 2BC but Not 3A Blocks Endoplasmic Reticulum-to-Golgi Transport. J. Virol. 79: 4382-4395 [Abstract] [Full Text]  
  • Jouvenet, N., Wileman, T. (2005). African swine fever virus infection disrupts centrosome assembly and function. J. Gen. Virol. 86: 589-594 [Abstract] [Full Text]  
  • Netherton, C. L., Parsley, J. C., Wileman, T. (2004). African Swine Fever Virus Inhibits Induction of the Stress-Induced Proapoptotic Transcription Factor CHOP/GADD153. J. Virol. 78: 10825-10828 [Abstract] [Full Text]  
  • Jouvenet, N., Monaghan, P., Way, M., Wileman, T. (2004). Transport of African Swine Fever Virus from Assembly Sites to the Plasma Membrane Is Dependent on Microtubules and Conventional Kinesin. J. Virol. 78: 7990-8001 [Abstract] [Full Text]  
  • Rodriguez, J. M., Garcia-Escudero, R., Salas, M. L., Andres, G. (2004). African Swine Fever Virus Structural Protein p54 Is Essential for the Recruitment of Envelope Precursors to Assembly Sites. J. Virol. 78: 4299-4313 [Abstract] [Full Text]  
  • Netherton, C., Rouiller, I., Wileman, T. (2004). The Subcellular Distribution of Multigene Family 110 Proteins of African Swine Fever Virus Is Determined by Differences in C-Terminal KDEL Endoplasmic Reticulum Retention Motifs. J. Virol. 78: 3710-3721 [Abstract] [Full Text]  
  • Husain, M., Moss, B. (2003). Evidence against an Essential Role of COPII-Mediated Cargo Transport to the Endoplasmic Reticulum-Golgi Intermediate Compartment in the Formation of the Primary Membrane of Vaccinia Virus. J. Virol. 77: 11754-11766 [Abstract] [Full Text]  
  • Alejo, A., Andres, G., Salas, M. L. (2003). African Swine Fever Virus Proteinase Is Essential for Core Maturation and Infectivity. J. Virol. 77: 5571-5577 [Abstract] [Full Text]  
  • Heath, C. M., Windsor, M., Wileman, T. (2003). Membrane Association Facilitates the Correct Processing of pp220 during Production of the Major Matrix Proteins of African Swine Fever Virus. J. Virol. 77: 1682-1690 [Abstract] [Full Text]  
  • Andres, G., Alejo, A., Salas, J., Salas, M. L. (2002). African Swine Fever Virus Polyproteins pp220 and pp62 Assemble into the Core Shell. J. Virol. 76: 12473-12482 [Abstract] [Full Text]  
  • Andres, G., Garcia-Escudero, R., Salas, M. L., Rodriguez, J. M. (2002). Repression of African Swine Fever Virus Polyprotein pp220-Encoding Gene Leads to the Assembly of Icosahedral Core-Less Particles. J. Virol. 76: 2654-2666 [Abstract] [Full Text]  
  • Cartwright, J. L., Safrany, S. T., Dixon, L. K., Darzynkiewicz, E., Stepinski, J., Burke, R., McLennan, A. G. (2002). The g5R (D250) Gene of African Swine Fever Virus Encodes a Nudix Hydrolase That Preferentially Degrades Diphosphoinositol Polyphosphates. J. Virol. 76: 1415-1421 [Abstract] [Full Text]  
  • McCrossan, M., Windsor, M., Ponnambalam, S., Armstrong, J., Wileman, T. (2001). The trans Golgi Network Is Lost from Cells Infected with African Swine Fever Virus. J. Virol. 75: 11755-11765 [Abstract] [Full Text]  
  • Griffiths, G., Roos, N., Schleich, S., Locker, J. K. (2001). Structure and Assembly of Intracellular Mature Vaccinia Virus: Thin-Section Analyses. J. Virol. 75: 11056-11070 [Abstract] [Full Text]  
  • Andres, G., Garcia-Escudero, R., Vinuela, E., Salas, M. L., Rodriguez, J. M. (2001). African Swine Fever Virus Structural Protein pE120R Is Essential for Virus Transport from Assembly Sites to Plasma Membrane but Not for Infectivity. J. Virol. 75: 6758-6768 [Abstract] [Full Text]  
  • Heath, C. M., Windsor, M., Wileman, T. (2001). Aggresomes Resemble Sites Specialized for Virus Assembly. JCB 153: 449-456 [Abstract] [Full Text]  
  • Lee, M. S., Zhu, Y. L., Sun, Z., Rhee, H., Jeromin, A., Roder, J., Dannies, P. S. (2000). Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 141: 3485-3492 [Abstract] [Full Text]  
  • Alejo, A., Andres, G., Vinuela, E., Salas, M. L. (1999). The African Swine Fever Virus Prenyltransferase Is an Integral Membrane trans-Geranylgeranyl-diphosphate Synthase. J. Biol. Chem. 274: 18033-18039 [Abstract] [Full Text]  
  • Kikkert, M., Van Lent, J., Storms, M., Bodegom, P., Kormelink, R., Goldbach, R. (1999). Tomato Spotted Wilt Virus Particle Morphogenesis in Plant Cells. J. Virol. 73: 2288-2297 [Abstract] [Full Text]  
  • Andres, G., Garcia-Escudero, R., Simon-Mateo, C., Vinuela, E. (1998). African Swine Fever Virus Is Enveloped by a Two-Membraned Collapsed Cisterna Derived from the Endoplasmic Reticulum. J. Virol. 72: 8988-9001 [Abstract] [Full Text]  
  • Cobbold, C., Wileman, T. (1998). The Major Structural Protein of African Swine Fever Virus, p73, Is Packaged into Large Structures, Indicative of Viral Capsid or Matrix Precursors, on the Endoplasmic Reticulum. J. Virol. 72: 5215-5223 [Abstract] [Full Text]  
  • Tait, S. W. G., Reid, E. B., Greaves, D. R., Wileman, T. E., Powell, P. P. (2000). Mechanism of Inactivation of NF-kappa B by a Viral Homologue of Ikappa Balpha . SIGNAL-INDUCED RELEASE OF Ikappa Balpha RESULTS IN BINDING OF THE VIRAL HOMOLOGUE TO NF-kappa B. J. Biol. Chem. 275: 34656-34664 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»