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J Virol, March 1998, p. 2208-2212, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cytosolic Gag p24 as an Index of Productive Entry of Human Immunodeficiency Virus Type 1

Valérie Maréchal,1 François Clavel,2 Jean Michel Heard,1 and Olivier Schwartz1,*

Laboratoire Rétrovirus et Transfert Génétique1 and Unité d'oncologie Virale,2 CNRS URA 1157, Institut Pasteur, 75724 Paris, France

Received 1 August 1997/Accepted 10 November 1997

We have investigated the cellular uptake of Gag p24 shortly after exposure of cells to human immunodeficiency virus (HIV) particles. In the absence of envelope glycoprotein on virions or of viral receptors or coreceptors at the cell surface, p24 was incorporated in intracellular vesicles but not detected in the cytosolic subcellular fraction. When appropriate envelope-receptor interactions could occur, the nonspecific vesicular uptake was still intense and cytosolic p24 represented 10 to 40% of total intracellular p24. The measurement of cytosolic p24 early after exposure to HIV type 1 is a reliable assay for investigating virus entry and early events leading to authentic cell infection.

* Corresponding author. Mailing address: Laboratoire Rétrovirus et Transfert Génétique, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 83 53. Fax: 33 1 45 68 89 40. E-mail: schwartz{at}pasteur.fr.




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