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J Virol, March 1998, p. 2160-2167, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Intercapsomeric Disulfide Bonds in Papillomavirus Assembly and Disassembly

Maolin Li,1 Peter Beard,2 Patricia A. Estes,1 Mary K. Lyon,3 and Robert L. Garcea1,*

Section of Pediatric Hematology/Oncology, Department of Pediatrics, University of Colorado School of Medicine, Denver, Colorado 802621; Swiss Institute for Experimental Cancer Research, 1066-Epalinges, Switzerland2; and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 803093

Received 23 September 1997/Accepted 26 November 1997

In order to analyze bonding contacts that stabilize the virion or promote capsid assembly, bovine papillomavirus (BPV) virions were subjected to buffer conditions known to disrupt polyomavirus virions. At physiologic ionic strength, incubation with dithiothreitol (DTT), EGTA, or DTT plus EGTA did not disrupt BPV virions as determined by electron microscopy. However, incubation of virions with DTT rendered the BPV L1 protein susceptible to trypsin cleavage at its carboxy terminus and rendered the genome susceptible to digestion with DNase I. When DTT-treated BPV virions were analyzed by analytical ultracentrifugation, they sedimented at 230S compared with 273S for untreated virions, suggesting a capsid shell expansion. Incubation with EGTA had no effect on trypsin or DNase I sensitivity and only a small effect upon the virion S value. A single cysteine residue conserved among BPV and human papillomavirus (HPV) L1 proteins resides within the trypsin-sensitive carboxy terminus of L1, which is required for capsid assembly. A recombinant HPV type 11 L1 protein, which was purified after expression in Escherichia coli and which has a Cys-to-Gly change at this position (Cys424), formed pentamers; however, unlike the wild-type protein, these mutant pentamers could no longer assemble in vitro into capsid-like structures. These results indicate an important role for interpentamer disulfide bonds in papillomavirus capsid assembly and disassembly and suggest a mechanism of virus uncoating in the reducing environment of the cytoplasm.

* Corresponding author. Mailing address: Section of Pediatric Hematology/Oncology, University of Colorado Health Sciences Center, Box C229, 4200 E. Ninth Ave., Denver, CO 80262.




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